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Journal / Developmental Biology / 5-mc נקודה כתם Assay לכמת את מתילציה DNA רמת…
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Developmental Biology

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JoVE Journal Developmental Biology
5-mc נקודה כתם Assay לכמת את מתילציה DNA רמת Chondrocyte Dedifiationiation
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5-mc נקודה כתם Assay לכמת את מתילציה DNA רמת Chondrocyte Dedifiationiation

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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אנו מציגים שיטה לכמת מתילציה דנ"א מבוסס על 5-methylcytosine (5-mC) כתם נקודה. קבענו את רמות 5-mC במהלך dedondferentiation chondrocyte. טכניקה פשוטה זו יכולה לשמש כדי לקבוע במהירות את הפנוטיפ chondrocyte בטיפול ACI.

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ביולוגיה התפתחותית גיליון 123 תרבות תאים הרחבת monolayer dedondferentiation chondrocyte מתילציה DNA 5-mC נקודה כתם ניתוח מיצוי DNA גנומי
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