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Journal / Genetics / Generasjon av innfødte Chromatin…
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Genetics

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JoVE Journal Genetics
Generasjon av innfødte Chromatin Immunoprecipitation sekvensering biblioteker til Nucleosome tetthet analyse
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Generasjon av innfødte Chromatin Immunoprecipitation sekvensering biblioteker til Nucleosome tetthet analyse

Article DOI: 10.3791/56085-v • 10:05 min • December 12th, 2017
December 12th, 2017 •
1, 1, 1, 1, 1,2
1Department of Microbiology and Immunology, Michael Smith Laboratories Centre for High-Throughput Biology, University of British Columbia, 2Canada's Michael Smith Genome Science Center, BC Cancer Agency

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Vi presenterer en modifisert innfødt chromatin immunoprecipitation sekvensering (ChIP-seq) metodikk for generering av sekvens datasett egnet for en nucleosome tetthet ChIP-seq analytiske rammen integrere micrococcal nuclease (MNase) tilgjengelighet med histone endring målinger.

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Genetikk problemet 130 Epigenomics chromatin immunoprecipitation histone modifisering nucleosome tetthet mobilnettet heterogenitet micrococcal nuclease
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