Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / High-throughput Parallel Sequencing to Measure…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Journal (Immunology and Infection)
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
Journal (Immunology and Infection)
Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
 

High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection

Article DOI: 10.3791/56442-v • 11:50 min • December 18th, 2017
December 18th, 2017 •
1,2, 1,2, 1,2, 1,2,3,4
1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles

Chapters

Summary
Automatic Translation

We describe here a technique that combines transposon mutagenesis with high-throughput sequencing to identify and quantify transposon leptospiral mutants in tissues after a challenge of hamsters. This protocol can be used to screen mutants for survival and dissemination in animals and can also be applied to in vitro studies.

Tags

High-throughput Parallel Sequencing Fitness Measurement Leptospira Interrogans Transposon Insertion Mutants Golden Syrian Hamster Infection In Vivo Fitness Defects Virulence Genes Colonization Dissemination Survival Large Number Of Mutants Limited Number Of Animals Acute Leptospirosis Chronic Infection Models In Vitro Studies Filtration Unit Leptospira Culture E.coli Culture EMJH Plate DAP
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter