07:27Results: Use of Quantitative Real-time Reverse Transcription PCR with or without Pre-amplification to Measure the Absolute Levels of Plasma micro-RNAs
This report describes a protocol for measuring the absolute levels of plasma miRNA, using quantitative real-time reverse transcription PCR with or without pre-amplification. This protocol affords better understanding of the quantity of plasma miRNAs and allows qualitative assessment of corresponding data from different studies or laboratories.