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Journal / Biochemistry / Purification of Hepatocytes and Sinusoidal…
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Biochemistry

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JoVE Journal Biochemistry
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Article DOI: 10.3791/56993-v • 08:54 min • February 12th, 2018
February 12th, 2018 •
1, 1, 1, 1, 1, 1, 1
1Department of Biochemistry, University of Nebraska

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Summary
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The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.

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Mouse Liver Perfusion Hepatocytes Sinusoidal Endothelial Cells Purification Liver Cell Populations In Vitro Assays In Vivo Assays Cell Viability Portal Vein Catheter Forceps Polyester Sewing Thread Cell Purification Technique Hepatology Field
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