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JoVE Journal Neuroscience
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
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Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

Article DOI: 10.3791/58479-v • 08:30 min • January 18th, 2019
January 18th, 2019 •
1, 1, 2, 2, 2, 2, 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

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Summary
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Here, we describe the essential steps for whole-cell patch-clamp recordings made from substantia gelatinosa (SG) neurons in the in vitro spinal cord slice. This method allows the intrinsic membrane properties, synaptic transmission and morphological characterization of SG neurons to be studied.

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Acute Spinal Cord Slices Whole-cell Patch-clamp Recording Substantia Gelatinosa Neurons Sensory Transmission Nociceptive Regulation Chronic Pain Neuronal Preservation In-vivo Conditions Agar Block Transverse Sections Parasagittal Sections Transcardial Perfusion Sucrose-based ACSF Dissection Tools Incision On The Back Skin Rib Cage Lumbosacral Segment Glass Dish
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