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Journal / Medicine / Isolation and Purification of Murine Cardiac…
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Isolation and Purification of Murine Cardiac Pericytes
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Isolation and Purification of Murine Cardiac Pericytes

Article DOI: 10.3791/59571-v • 10:49 min • August 16th, 2019
August 16th, 2019 •
1, 2, 1
1Department of Cardiometabolic Disorders, Amgen Research and Discovery, Amgen Inc., 2Department of Translational Sciences, Amgen Research and Discovery, Amgen Inc.

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Summary
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We have optimized a protocol to isolate and purify murine cardiac pericytes for basic research and investigation of their biology and therapeutic potential.

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Isolation Purification Murine Cardiac Pericytes Mouse Model Protocol Materials Investigators Healthy Disease Genetically Altered Cardiac Pericyte Biology Mouse Model Of Disease Genetic Variation Insight Contribution Cardiac Homeostasis Hemodynamics Anesthetized Mouse Supine Position Chest Cavity Cannulate Descending Aorta Butterfly Needle Right Atrium Perfuse Heart Heparinized Calcium-magnesium-free Dulbecco's Phosphate Buffered Saline (DPBS) Milliliters Variable-flow Peristaltic Pump Petri Dish Enzyme Solution
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