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JoVE Journal Developmental Biology
Un metodo di montaggio a strati per la microscopia confocale Time-Lapse estesa di embrioni di pesci zebra interi
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Un metodo di montaggio a strati per la microscopia confocale Time-Lapse estesa di embrioni di pesci zebra interi

Article DOI: 10.3791/60321-v • 08:55 min • January 14th, 2020
January 14th, 2020 •
1, 2, 2, 3,4, 1,4, 5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University

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Questo articolo descrive un metodo per montare fragili embrioni di pesce zebra per la microscopia confocale time-lapse estesa. Questo metodo a basso costo è facile da eseguire utilizzando normali piatti di microscopia con fondo di vetro per l'imaging su qualsiasi microscopio invertito. Il montaggio viene eseguito in strati di agarose a diverse concentrazioni.

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Biologia dello sviluppo numero 155 pesce zebra embrione microscopia confocale time-lapse montaggio transgenico
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