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JoVE Journal Bioengineering
Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
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Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

Article DOI: 10.3791/60434-v • 07:55 min • December 13th, 2019
December 13th, 2019 •
*1, *2, 2, 3, 3,4, 1,2,5
1Department of Mechanical Engineering, Pohang University of Science and Technology, 2School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 3Asan Institute for Life Science, University of Ulsan College of Medicine and Asan Medical Center, 4Division of Hepato-Biliary and Pancreatic Surgery, Department of Surgery, University of Ulsan College of Medicine and Asan Medical Center, 5Department of Creative IT Engineering, Pohang University of Science and Technology
* These authors contributed equally

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Summary
Automatic Translation

Decellularized extracellular matrix (dECM) can provide suitable microenvironmental cues to recapitulate the inherent functions of target tissues in an engineered construct. This article elucidates the protocols for the decellularization of pancreatic tissue, evaluation of pancreatic tissue-derived dECM bioink, and generation of 3D pancreatic tissue constructs using a bioprinting technique.

Tags

Pancreatic Tissue Extracellular Matrix (ECM) Bioink 3D Printing Cell-laden Tissue Construct Pancreatic Islets Neurological Properties Cell Viability Tissue-specific Composition Islet Encapsulation Type One Diabetes Treatment In Vitro Tissue Models Diabetes-related Complications Pancreatic Cancer Decellularization Porcine Pancreas Tissue Sample
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