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JoVE Journal Bioengineering
Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Article DOI: 10.3791/60629-v • 10:52 min • January 19th, 2020
January 19th, 2020 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Public Health, University of Naples Federico II
* These authors contributed equally

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Summary
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Cell reprogramming requires the introduction of key genes, which regulate and maintain the pluripotent cell state. The protocol described enables the formation of induced pluripotent stem cells (iPSCs) colonies from human dermal fibroblasts without viral/integrating methods but using non-modified RNAs (NM-RNAs) combined with immune evasion factors reducing cellular defense mechanisms.

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Isolation Adult Human Dermal Fibroblasts Abdominal Skin Induced Pluripotent Stem Cells Non-Integrating Method Autologous Reprogramming Skin Punch Biopsy Xeno-free Regenerative Medicine Genetic Disorders Cancer Modeling Molecular Mechanisms Specific Therapies Workload Experiment Planning Cell Isolation Reprogramming Microbial Contamination RNA Contamination Sterile Hood HPSS Solution Fine Forceps Scissors Scalpel
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