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High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
JoVE Journal
Biology
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JoVE Journal Biology
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

DOI:
10.3791/60800-v

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08:18 min

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June 16, 2020

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, , , ,
1Advanced ICT Research Institute Kobe,National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences,Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry,University of Oxford

Chapters

  • 00:04Introduction
  • 00:40Sample Preparation for Imaging
  • 02:50Acquisition of Reference Images
  • 04:47Correction of Chromatic Shift Using Chromagnon Software
  • 06:25Results: Chromatic Shift Correction with Chromagnon
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

Tags

3D Chromatic ShiftChromatic Aberration CorrectionSuper-resolution MicroscopyBiological ImagingChromagnonColocalization AnalysisWidefield MicroscopyFluorescent StainingPhalloidinAlexa FluorMounting Medium

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