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High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
JoVE Journal
Biology
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JoVE Journal Biology
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
DOI:

08:18 min

June 16, 2020

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Chapters

  • 00:04Introduction
  • 00:40Sample Preparation for Imaging
  • 02:50Acquisition of Reference Images
  • 04:47Correction of Chromatic Shift Using Chromagnon Software
  • 06:25Results: Chromatic Shift Correction with Chromagnon
  • 07:51Conclusion

Summary

Automatic Translation

Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

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