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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy

Article DOI: 10.3791/60934-v • 10:37 min • March 16th, 2020
March 16th, 2020 •
1, 1, 1,3, 2, 1,3
1Institute of Physical Chemistry, Albert-Ludwigs-Universität Freiburg, 2Chair in Polymer Chemistry, Saarland University, 3Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg

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Summary
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Covalent attachment of probe molecules to atomic force microscopy (AFM) cantilever tips is an essential technique for the investigation of their physical properties. This allows us to determine the stretching force, desorption force and length of polymers via AFM-based single molecule force spectroscopy with high reproducibility.

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AFM-based Force Spectroscopy Single Molecule Covalent Attachment Binding Protocol Polymers Mechanical Properties Adhesive Properties AFM Cantilever Tip Fume Hood Solvent-resistant Glass Plasma Chamber Surface Activation Program Plasma Process Silane-PEG-mal Toluene Concentration Petri Dish Incubate Contaminants
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