Articles by Anke Van der Perren in JoVE

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model

JoVE 4219 10/31/2012

Marianne S. Carlon*¹, Jaan Toelen*², Marina Mori da Cunha², Dragana Vidović¹, Anke Van der Perren³, Steffi Mayer², Lourenço Sbragia², Johan Nuyts⁴, Uwe Himmelreich⁵, Zeger Debyser¹, Jan Deprest²

¹Molecular Virology and Gene Therapy, KU Leuven, ²Department of Woman and Child, KU Leuven, ³Neurobiology and Gene Therapy, KU Leuven, ⁴Division of Nuclear Medicine, KU Leuven, ⁵Biomedical NMR Unit/ MoSAIC, KU Leuven

We developed a novel surgical approach for intratracheal administration of bioactive agents into the mouse fetus. The delivery route is more efficient in targeting the fetal mouse lungs than the commonly used intra-amniotic injection. This procedure has to date not been described in a mouse model.