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In JoVE (1)
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Articles by Carol E. Soteropulos in JoVE
JoVE Bioengineering
Bevestiging van biologische Probes aan Silica optische biosensoren gebruik van silaan agenten
Department of Biological Engineering, University of Missouri
Biosensoren-interface met complexe, biologische omgevingen en het uitvoeren van gerichte opsporing door het combineren van zeer gevoelige sensoren met zeer specifieke probes verbonden aan de sensor via oppervlakte modificatie. Hier tonen we het oppervlak functionalisering van silica optische sensoren met biotine met silaan koppelingsmiddelen aan de sensor en de biologische omgeving overbruggen.
Other articles by Carol E. Soteropulos on PubMed
Determination of Binding Kinetics Using Whispering Gallery Mode Microcavities
Silica optical microcavity sensors show great promise in the kinetic evaluation of binding pairs, fundamental in understanding biomolecular interactions. Here, we develop and demonstrate a novel platform, based on bioconjugated silica microsphere resonators, to study the binding kinetics of the biotin-streptavidin system. We characterize the optical performance, verify the covalent attachment of biotin to the surface, and perform streptavidin detection experiments. We perform preliminary kinetic analysis of the detection data which shows the potential of whispering gallery mode resonators in the determination of the dissociation constant of the binding pair, which is in good agreement with previously published values.
