Articles by Christine V. Portfors in JoVE

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers

JoVE 3755 6/26/2012

Michael A. Muniak^1,2, Zachary M. Mayko³, David K. Ryugo^2,4, Christine V. Portfors³

¹Department of Neuroscience, Johns Hopkins University, ²Garvan Institute of Medical Research, ³School of Biological Sciences, Washington State University, ⁴Department of Otolaryngology-HNS, Johns Hopkins University

Electrophysiological characterization of neuronal responses is important for understanding brain function and for guiding the placement of dyes for pathway tracing. However, many studies are performed in anesthetized animals. To understand brain function without anesthetics, we developed a method to record neuronal response properties and inject dyes in awake mouse.