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In JoVE (1)
- Engenharia e Evolução da Synthetic vírus adeno-associado (AAV) Gene vetores de terapia através de DNA Família Shuffling
Other Publications (1)
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Articles by Eike Kienle in JoVE
Engenharia e Evolução da Synthetic vírus adeno-associado (AAV) Gene vetores de terapia através de DNA Família Shuffling
Eike Kienle*1, Elena Senís*1, Kathleen Börner2, Dominik Niopek1, Ellen Wiedtke1, Stefanie Grosse1, Dirk Grimm1
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
Nós demonstramos a base técnica para projetar e evoluir molecularmente sintéticos virais adeno-associados (AAV) vetores de terapia genética através de DNA da família baralhar. Além disso, fornecer orientações gerais e exemplos representativos para a selecção ea análise de individuais cápsides quiméricos com propriedades melhoradas sobre células alvo em cultura ou em ratinhos.
Other articles by Eike Kienle on PubMed
CLEC2A: a Novel, Alternatively Spliced and Skin-associated Member of the NKC-encoded AICL-CD69-LLT1 Family
Immunogenetics. Dec, 2007 | Pubmed ID: 18046548
The human natural killer gene complex (NKC) encodes for numerous C-type lectin-like receptors (CTLR), which are expressed on various immune cells including natural killer (NK) cells and myeloid cells. Certain activation-induced, NKC-encoded CTLR are grouped into the C-type lectin domain family 2 (CLEC2 family) which, in humans, comprises AICL (CLEC2B), CD69 (CLEC2C), and LLT1 (CLEC2D). In this paper, we characterize a novel member of the CLEC2 family, the human orphan gene CLEC2A. The C-type lectin-like domain (CTLD) of CLEC2A is most similar to the CTLD of LLT1 ( approximately 60% similarity). Like mouse CLEC2 family members Clr-b and Clr-g, CLEC2A lacks two highly conserved cysteines (Cys4 and Cys5), which form an intramolecular bond in the CTLD of most CTLR. Alternative splicing of exon 2 and of two distinct terminal exons (exon 5A/B), respectively, gives rise to four CLEC2A variants differing in the usage of the transmembrane domain and/or in the carboxyterminal portion of the CTLD. CLEC2A transcripts were detected primarily in myeloid cell lines, but not in epithelial cell lines. In tissues, CLEC2A is selectively expressed in the skin and, at lower abundance, in hematopoietic and gonadal tissues. Finally, we show that the CLEC2A1 variant is readily expressed at the cell surface, where it may serve as a ligand for NKC-encoded NK receptors.