Articles by Elli A. Wright in JoVE

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

JoVE 3857 6/05/2012

Sebastian Kirchner¹, Joanne L Fothergill², Elli A. Wright¹, Chloe E. James¹, Eilidh Mowat¹, Craig Winstanley¹

¹Institute of Infection and Global Health, University of Liverpool, ²NIHR Biomedical Research Centre in Microbial Disease, University of Liverpool

Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.