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JoVE Immunology and Infection Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens JoVE 2221 11/23/2010 Isfahan R. Chambers*1, Tiffany R. Cone*1, Kyra Oswald-Richter2, Wonder P. Drake1,2 1Department of Medicine, Vanderbilt University School of Medicine, 2Department of Microbiology and Immunology, Vanderbilt University School of Medicine Identification of microbial targets of adaptive immunity in idiopathic diseases can be accomplished by the use of the enzyme-linked immunospot assay.