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In JoVE (4)
- Dissection of Human Vitreous Body Elements for Proteomic Analysis
- Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
- Mouse Eye Enucleation for Remote High-throughput Phenotyping
- Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
Other Publications (9)
Articles by Jessica M. Skeie in JoVE
Dissection of Human Vitreous Body Elements for Proteomic Analysis
Jessica M. Skeie, Vinit B. Mahajan
Department of Ophthalmology and Visual Sciences, Omics Laboratory, University of Iowa
This video shows an effective technique for differentiating and dissecting the various semi-transparent structures of the human vitreous body in post mortem eyes.
Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
Jessica M. Skeie1,2, Stephen H. Tsang3, Vinit B. Mahajan1,2
1Omics Laboratory, University of Iowa, 2Ophthalmology and Visual Sciences, University of Iowa, 3Harkness Eye Institute, Columbia University College of Physicians and Surgeons
The dissection technique illustrates evisceration of the vitreous, retina, and lens from the mouse eye, separation by centrifugation, and characterization with protein assays.
Mouse Eye Enucleation for Remote High-throughput Phenotyping
Vinit B. Mahajan1,2, Jessica M. Skeie1,2, Amir H. Assefnia2,3, MaryAnn Mahajan1,2, Stephen H. Tsang2,4
1Department of Ophthalmology and Visual Sciences, University of Iowa, 2Omics Laboratory, University of Iowa, 3School of Dentistry, UCLA, 4Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University
The dissection technique illustrates enucleation of the mouse eye for tissue fixation to perform phenotyping in high-throughput screens.
Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
Katherine J. Wert1,2, Jessica M. Skeie3,4, Richard J. Davis1, Stephen H. Tsang1,3, Vinit B. Mahajan3,4
1Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, Columbia University, 2Institute of Human Nutrition, College of Physicians & Surgeons, Columbia University, 3Omics Laboratory, University of Iowa, 4Department of Ophthalmology and Visual Sciences, University of Iowa
This surgical technique illustrates the injection of gene therapy vectors and stem cells into the subretinal space of the mouse eye.
Other articles by Jessica M. Skeie on PubMed
Molecular Vision. Jul, 2005 | Pubmed ID: 16052166
Choroidal neovascularization (CNV) is a complication of multiple eye diseases, including age-related macular degeneration, that usually results in irreversible vision loss. It is characterized by proliferation and growth of choroidal blood vessels through Bruch's membrane into the subpigment epithelial and/or subretinal space. The purpose of this study was to characterize the carbohydrate groups associated with CNV by lectin histochemistry.
Molecular Vision. 2006 | Pubmed ID: 16604055
In order to understand the extent of choriocapillary endothelial cell activation in different topographic regions of the eye, we sought to compare the localization of intercellular adhesion molecule-1 (ICAM-1) in macular and peripheral regions of human eyes.
Comparison of the Femtosecond Laser (IntraLase) Versus Manual Microkeratome (Moria ALTK) in Dissection of the Donor in Endothelial Keratoplasty: Initial Study in Eye Bank Eyes
Cornea. Jan, 2008 | Pubmed ID: 18245973
To determine the safety and efficacy of a femtosecond laser (IntraLase) and manual microkeratome (Moria ALTK) in creating precut endothelial keratoplasty donor tissue.
Elastin-mediated Choroidal Endothelial Cell Migration: Possible Role in Age-related Macular Degeneration
Investigative Ophthalmology & Visual Science. Dec, 2008 | Pubmed ID: 18708613
Endothelial cell (EC) migration is a key event in angiogenesis, and is likely to play an important role in choroidal neovascularization in age-related macular degeneration (AMD). Altered elastin metabolism has been described in AMD, and the present study sought to determine the effects of elastin-derived peptides (EDPs) on choroidal EC migration and proliferation.
Investigative Ophthalmology & Visual Science. Oct, 2010 | Pubmed ID: 20484595
The complement system plays a crucial role in the progression of age-related macular degeneration (AMD). In this study, the authors sought to evaluate the pathophysiologic roles of complement components C3a and C5a in the human choroid in AMD.
Molecular Vision. 2011 | Pubmed ID: 21364907
Age-related macular degeneration (AMD) is a common blinding disease in the elderly population. AMD is frequently complicated by choroidal neovascularization, causing irreversible losses in visual acuity. Proteins that induce pathologic angiogenesis in other systems include angiogenin, a small protein involved in angiogenesis in tumor metastases. Our goal was to determine if angiogenin participates in angiogenesis during choroidal neovascular membrane formation in AMD.
Choriocapillaris Vascular Dropout Related to Density of Drusen in Human Eyes with Early Age-related Macular Degeneration
Investigative Ophthalmology & Visual Science. Mar, 2011 | Pubmed ID: 21398287
Age-related macular degeneration (AMD) is a common, potentially blinding disease characterized by the presence of extracellular deposits beneath the retinal pigment epithelium (RPE). Choroidal vascular changes have also been noted in AMD. This study examined the relationship between the choroidal vasculature and extent of drusen and other sub-RPE deposits, the key pathologic landmarks of AMD.
BMC Medical Genetics. 2011 | Pubmed ID: 21521525
Age-related macular degeneration (AMD) is a common disease of the elderly that leads to loss of the central visual field due to atrophic or neovascular events. Evidence from human eyes and animal models suggests an important role for macrophages and endothelial cell activation in the pathogenesis of AMD. We sought to determine whether common ancestral variants in genes encoding the selectin family of proteins are associated with AMD.
Molecular Responses of Choroidal Endothelial Cells to Elastin Derived Peptides Through the Elastin-binding Protein (GLB1)
Matrix Biology : Journal of the International Society for Matrix Biology. Dec, 2011 | Pubmed ID: 22178079
PURPOSE: Neovascular AMD involves the activation of choroidal endothelial cells to increase their inflammatory and angiogenic behaviors. Elastin derived peptides (EDPs) can elicit some of these phenotypic changes in endothelial cells. This investigation was performed to follow up on those findings by determining a receptor for these peptides in the human eye as well as evaluating the effects of elevated EDPs on choroidal cells in vitro and in vivo. METHODS: The expression of elastin receptor genes including GLB1 was analyzed using reverse transcription PCR. Migration of choroidal endothelial cells was quantified in the presence of inhibitors to different EDP binding proteins. C57BL6 mice were injected with EDPs and studied by electroretinography, transmission electron microscopy, and microarray analysis. RESULTS: An alternatively spliced form of beta-galactosidase (GLB1) is present on human choroidal endothelial cells and acts as a receptor for EDPs. Elevated levels of circulating EDPs do not affect retinal function in the mouse, but do increase the expression and deposition of collagen IV in the RPE/choroid complex. CONCLUSIONS: EDPs may play a role in neovascular AMD by binding to and inducing neovascular phenotypes in choroidal endothelial cells through their receptor, GLB1. These peptides also cause an increased mRNA expression and deposition of collagen IV in the RPE/choroid, which may alter diffusion properties between the retina and choriocapillaris.