Jonathon Howard Department of Molecular Biophysics & Biochemistry Yale University Biography Publications Institution JoVE Articles Jonathon Howard has not added a biography. If you are Jonathon Howard and would like to personalize this page please email our Author Liaison for assistance. Publications In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Reflection Microscopy Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 35476326 Counting Fluorescently Labeled Proteins in Tissues in the Spinning Disk Microscope Using Single-molecule Calibrations Molecular Biology of the Cell. Mar, 2022 | Pubmed ID: 35323029 Structures of Outer-arm Dynein Array on Microtubule Doublet Reveal a Motor Coordination Mechanism Nature Structural & Molecular Biology. 10, 2021 | Pubmed ID: 34556869 The Narrowing of Dendrite Branches Across Nodes Follows a Well-defined Scaling Law Proceedings of the National Academy of Sciences of the United States of America. 07, 2021 | Pubmed ID: 34215693 Focal Laser Stimulation of Fly Nociceptors Activates Distinct Axonal and Dendritic Ca Signals Biophysical Journal. 08, 2021 | Pubmed ID: 34175294 Physical Bioenergetics: Energy Fluxes, Budgets, and Constraints in Cells Proceedings of the National Academy of Sciences of the United States of America. 06, 2021 | Pubmed ID: 34140336 Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes Trends in Cell Biology. 01, 2021 | Pubmed ID: 33183955 Purification of Ciliary Tubulin from Chlamydomonas Reinhardtii Current Protocols in Protein Science. 06, 2020 | Pubmed ID: 32568459 Contribution of Increasing Plasma Membrane to the Energetic Cost of Early Zebrafish Embryogenesis Molecular Biology of the Cell. 03, 2020 | Pubmed ID: 32049586 Three Beads Are Better Than One Biophysical Journal. 01, 2020 | Pubmed ID: 31951531 Predicted Effects of Severing Enzymes on the Length Distribution and Total Mass of Microtubules Biophysical Journal. 12, 2019 | Pubmed ID: 31708162 The Kinetics of Nucleotide Binding to Isolated Chlamydomonas Axonemes Using UV-TIRF Microscopy Biophysical Journal. 08, 2019 | Pubmed ID: 31400919 Nicotinamide Adenine Dinucleotides and Their Precursor NMN Have No Direct Effect on Microtubule Dynamics in Purified Brain Tubulin PloS One. 2019 | Pubmed ID: 31393939 Force Generated by Two Kinesin Motors Depends on the Load Direction and Intermolecular Coupling Physical Review Letters. May, 2019 | Pubmed ID: 31144901 The Dynamic and Structural Properties of Axonemal Tubulins Support the High Length Stability of Cilia Nature Communications. 04, 2019 | Pubmed ID: 31015426 Spastin is a Dual-function Enzyme That Severs Microtubules and Promotes Their Regrowth to Increase the Number and Mass of Microtubules Proceedings of the National Academy of Sciences of the United States of America. 03, 2019 | Pubmed ID: 30837315 Heat Oscillations Driven by the Embryonic Cell Cycle Reveal the Energetic Costs of Signaling Developmental Cell. Mar, 2019 | Pubmed ID: 30713074 Label-free High-speed Wide-field Imaging of Single Microtubules Using Interference Reflection Microscopy Journal of Microscopy. Oct, 2018 | Pubmed ID: 30044498 Structural Biology: Piezo Senses Tension Through Curvature Current Biology : CB. Apr, 2018 | Pubmed ID: 29689211 Physical Limits on the Precision of Mitotic Spindle Positioning by Microtubule Pushing Forces: Mechanics of Mitotic Spindle Positioning BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology. 11, 2017 | Pubmed ID: 28960439 Structural Biology: A Force-Sensitive Ion Channel Springs to Life Current Biology : CB. 09, 2017 | Pubmed ID: 28950085 Curvature Regulation of the Ciliary Beat Through Axonemal Twist Physical Review. E. Oct, 2016 | Pubmed ID: 27841522 Mechanism of Microtubule Lumen Entry for the α-tubulin Acetyltransferase Enzyme αTAT1 Proceedings of the National Academy of Sciences of the United States of America. 11, 2016 | Pubmed ID: 27803321 The Mitotic Spindle in the One-Cell C. Elegans Embryo Is Positioned with High Precision and Stability Biophysical Journal. Oct, 2016 | Pubmed ID: 27760363 A Force-generating Machinery Maintains the Spindle at the Cell Center During Mitosis Science (New York, N.Y.). May, 2016 | Pubmed ID: 27230381 Dynamic Curvature Regulation Accounts for the Symmetric and Asymmetric Beats of Chlamydomonas Flagella ELife. 05, 2016 | Pubmed ID: 27166516 Broken Detailed Balance at Mesoscopic Scales in Active Biological Systems Science (New York, N.Y.). Apr, 2016 | Pubmed ID: 27126047 Automatic Optimal Filament Segmentation with Sub-pixel Accuracy Using Generalized Linear Models and B-spline Level-sets Medical Image Analysis. 08, 2016 | Pubmed ID: 27104582 Microtubules: 50 Years on from the Discovery of Tubulin Nature Reviews. Molecular Cell Biology. 04, 2016 | Pubmed ID: 27103327 Independent Control of the Static and Dynamic Components of the Chlamydomonas Flagellar Beat Current Biology : CB. 04, 2016 | Pubmed ID: 27040779 Splicing of Nascent RNA Coincides with Intron Exit from RNA Polymerase II Cell. Apr, 2016 | Pubmed ID: 27020755 Regulation of Microtubule Growth and Catastrophe: Unifying Theory and Experiment Trends in Cell Biology. Dec, 2015 | Pubmed ID: 26616192 Kinesin Kip2 Enhances Microtubule Growth in Vitro Through Length-dependent Feedback on Polymerization and Catastrophe ELife. Nov, 2015 | Pubmed ID: 26576948 The Motility of Axonemal Dynein is Regulated by the Tubulin Code Biophysical Journal. Dec, 2014 | Pubmed ID: 25658008 The Microtubule-based Cytoskeleton is a Component of a Mechanical Signaling Pathway in Fly Campaniform Receptors Biophysical Journal. Dec, 2014 | Pubmed ID: 25517144 Quantitative Cell Biology: the Essential Role of Theory Molecular Biology of the Cell. Nov, 2014 | Pubmed ID: 25368416 Stu2, the Budding Yeast XMAP215/Dis1 Homolog, Promotes Assembly of Yeast Microtubules by Increasing Growth Rate and Decreasing Catastrophe Frequency The Journal of Biological Chemistry. Oct, 2014 | Pubmed ID: 25172511 Motor Regulation Results in Distal Forces That Bend Partially Disintegrated Chlamydomonas Axonemes into Circular Arcs Biophysical Journal. Jun, 2014 | Pubmed ID: 24896122 A Brief Scientific Biography of Prof. Alan J. Hunt Cellular and Molecular Bioengineering. Dec, 2013 | Pubmed ID: 24683426 Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins Yazgan Tuna1, Amer Al-Hiyasat1, Jonathon Howard1,2 1Department of Molecular Biophysics & Biochemistry, Yale University, 2Department of Physics, Yale University JoVE 63730 Biochemistry Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules Mohammed Mahamdeh1,2, Jonathon Howard1 1Department of Molecular Biophysics and Biochemistry, Yale University, 2Harvard Medical School, Harvard University JoVE 59520 Bioengineering
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins Yazgan Tuna1, Amer Al-Hiyasat1, Jonathon Howard1,2 1Department of Molecular Biophysics & Biochemistry, Yale University, 2Department of Physics, Yale University JoVE 63730 Biochemistry
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules Mohammed Mahamdeh1,2, Jonathon Howard1 1Department of Molecular Biophysics and Biochemistry, Yale University, 2Harvard Medical School, Harvard University JoVE 59520 Bioengineering
