Kevin Eggan Stanley Center for Psychiatric Research Broad Institute of MIT and Harvard Biography Publications Institution JoVE Articles Kevin Eggan has not added a biography. If you are Kevin Eggan and would like to personalize this page please email our Author Liaison for assistance. Publications A Multiplexed GRNA PiggyBac Transposon System Facilitates Efficient Induction of CRISPRi and CRISPRa in Human Pluripotent Stem Cells Scientific Reports. Jan, 2020 | Pubmed ID: 31959800 Publisher Correction: Exome Sequencing in Amyotrophic Lateral Sclerosis Implicates a Novel Gene, DNAJC7, Encoding a Heat-shock Protein Nature Neuroscience. Feb, 2020 | Pubmed ID: 31857710 Herpesviral Lytic Gene Functions Render the Viral Genome Susceptible to Novel Editing by CRISPR/Cas9 ELife. 12, 2019 | Pubmed ID: 31789594 Exome Sequencing in Amyotrophic Lateral Sclerosis Implicates a Novel Gene, DNAJC7, Encoding a Heat-shock Protein Nature Neuroscience. 12, 2019 | Pubmed ID: 31768050 RNA-seq As a Tool for Evaluating Human Embryo Competence Genome Research. 10, 2019 | Pubmed ID: 31548358 Cancer-Related Mutations Identified in Primed and Naive Human Pluripotent Stem Cells Cell Stem Cell. Oct, 2019 | Pubmed ID: 31543368 A High-Content Screen Identifies TPP1 and Aurora B As Regulators of Axonal Mitochondrial Transport Cell Reports. Sep, 2019 | Pubmed ID: 31533043 Comparison of Three Congruent Patient-specific Cell Types for the Modelling of a Human Genetic Schwann-cell Disorder Nature Biomedical Engineering. 07, 2019 | Pubmed ID: 30962586 Dysregulated Protocadherin-pathway Activity As an Intrinsic Defect in Induced Pluripotent Stem Cell-derived Cortical Interneurons from Subjects with Schizophrenia Nature Neuroscience. 02, 2019 | Pubmed ID: 30664768 ALS-implicated Protein TDP-43 Sustains Levels of STMN2, a Mediator of Motor Neuron Growth and Repair Nature Neuroscience. 02, 2019 | Pubmed ID: 30643292 A Stem Cell-Based Screening Platform Identifies Compounds That Desensitize Motor Neurons to Endoplasmic Reticulum Stress Molecular Therapy : the Journal of the American Society of Gene Therapy. 01, 2019 | Pubmed ID: 30446391 Convergence of Independent DISC1 Mutations on Impaired Neurite Growth Via Decreased UNC5D Expression Translational Psychiatry. 11, 2018 | Pubmed ID: 30410030 Oligodendrocyte Differentiation of Induced Pluripotent Stem Cells Derived from Subjects with Schizophrenias Implicate Abnormalities in Development Translational Psychiatry. 10, 2018 | Pubmed ID: 30352993 Comparative Genomic Analysis of Embryonic, Lineage-converted and Stem Cell-derived Motor Neurons Development (Cambridge, England). 11, 2018 | Pubmed ID: 30337375 All-optical Synaptic Electrophysiology Probes Mechanism of Ketamine-induced Disinhibition Nature Methods. 10, 2018 | Pubmed ID: 30275587 Hippo Signaling in the Ovary and Polycystic Ovarian Syndrome Journal of Assisted Reproduction and Genetics. Oct, 2018 | Pubmed ID: 30120633 Dipeptide Repeat Proteins Activate a Heat Shock Response Found in C9ORF72-ALS/FTLD Patients Acta Neuropathologica Communications. 07, 2018 | Pubmed ID: 29973287 The C9orf72-interacting Protein Smcr8 is a Negative Regulator of Autoimmunity and Lysosomal Exocytosis Genes & Development. 07, 2018 | Pubmed ID: 29950492 TDP-43 Induces P53-mediated Cell Death of Cortical Progenitors and Immature Neurons Scientific Reports. 05, 2018 | Pubmed ID: 29802307 Combining NGN2 Programming with Developmental Patterning Generates Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission Cell Reports. 05, 2018 | Pubmed ID: 29791859 All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human IPSC-Derived Motor Neuron Model of ALS Stem Cell Reports. 06, 2018 | Pubmed ID: 29779896 Publisher Correction: Whole Genome Sequencing in Psychiatric Disorders: the WGSPD Consortium Nature Neuroscience. Jul, 2018 | Pubmed ID: 29549319 Whole Genome Sequencing in Psychiatric Disorders: the WGSPD Consortium Nature Neuroscience. 12, 2017 | Pubmed ID: 29184211 Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons Stem Cell Reports. 11, 2017 | Pubmed ID: 29107592 A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease Stem Cell Reports. 10, 2017 | Pubmed ID: 29020615 Reactive Astrocytes Promote ALS-like Degeneration and Intracellular Protein Aggregation in Human Motor Neurons by Disrupting Autophagy Through TGF-β1 Stem Cell Reports. Aug, 2017 | Pubmed ID: 28712846 Assessing the Safety of Human Pluripotent Stem Cells and Their Derivatives for Clinical Applications Stem Cell Reports. Jul, 2017 | Pubmed ID: 28700896 Human Pluripotent Stem Cells Recurrently Acquire and Expand Dominant Negative P53 Mutations Nature. 05, 2017 | Pubmed ID: 28445466 Genetic Ablation of AXL Does Not Protect Human Neural Progenitor Cells and Cerebral Organoids from Zika Virus Infection Cell Stem Cell. Dec, 2016 | Pubmed ID: 27912091 Generation of a TLE3 Heterozygous Knockout Human Embryonic Stem Cell Line Using CRISPR-Cas9 Stem Cell Research. Sep, 2016 | Pubmed ID: 27879221 Generation of a TLE1 Homozygous Knockout Human Embryonic Stem Cell Line Using CRISPR-Cas9 Stem Cell Research. Sep, 2016 | Pubmed ID: 27879218 Two Familial ALS Proteins Function in Prevention/repair of Transcription-associated DNA Damage Proceedings of the National Academy of Sciences of the United States of America. Nov, 2016 | Pubmed ID: 27849576 Monitoring Peripheral Nerve Degeneration in ALS by Label-free Stimulated Raman Scattering Imaging Nature Communications. Oct, 2016 | Pubmed ID: 27796305 Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells Current Protocols in Stem Cell Biology. Aug, 2016 | Pubmed ID: 27532820 Loss-of-function Mutations in the C9ORF72 Mouse Ortholog Cause Fatal Autoimmune Disease Science Translational Medicine. Jul, 2016 | Pubmed ID: 27412785 CAT7 and Cat7l Long Non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development The Journal of Biological Chemistry. Sep, 2016 | Pubmed ID: 27405765 Modeling ALS with Motor Neurons Derived from Human Induced Pluripotent Stem Cells Nature Neuroscience. Apr, 2016 | Pubmed ID: 27021939 SLC52A3, A Brown-Vialetto-van Laere Syndrome Candidate Gene is Essential for Mouse Development, but Dispensable for Motor Neuron Differentiation Human Molecular Genetics. May, 2016 | Pubmed ID: 26976849 A Perspective on Stem Cell Modeling of Amyotrophic Lateral Sclerosis Cell Cycle (Georgetown, Tex.). 2015 | Pubmed ID: 26505672 Automated, High-throughput Derivation, Characterization and Differentiation of Induced Pluripotent Stem Cells Nature Methods. Sep, 2015 | Pubmed ID: 26237226 From Dish to Bedside: Lessons Learned While Translating Findings from a Stem Cell Model of Disease to a Clinical Trial Cell Stem Cell. Jul, 2015 | Pubmed ID: 26140603 Efficient CRISPR-Cas9-mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus Cell Reports. May, 2015 | Pubmed ID: 25937281 Focus on Induced Pluripotency and Cellular Reprogramming The EMBO Journal. Jun, 2015 | Pubmed ID: 25916828 Generation of Neuropeptidergic Hypothalamic Neurons from Human Pluripotent Stem Cells Development (Cambridge, England). Feb, 2015 | Pubmed ID: 25670790 ALS-causative Mutations in FUS/TLS Confer Gain and Loss of Function by Altered Association with SMN and U1-snRNP Nature Communications. 2015 | Pubmed ID: 25625564 Motoneurons Derived from Induced Pluripotent Stem Cells Develop Mature Phenotypes Typical of Endogenous Spinal Motoneurons The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2015 | Pubmed ID: 25609642 Modeling Pain in Vitro Using Nociceptor Neurons Reprogrammed from Fibroblasts Nature Neuroscience. Jan, 2015 | Pubmed ID: 25420066 IPSC-derived Dopamine Neurons Reveal Differences Between Monozygotic Twins Discordant for Parkinson's Disease Cell Reports. Nov, 2014 | Pubmed ID: 25456120 Genetic Variation in Human DNA Replication Timing Cell. Nov, 2014 | Pubmed ID: 25416942 The Role of Maternal-specific H3K9me3 Modification in Establishing Imprinted X-chromosome Inactivation and Embryogenesis in Mice Nature Communications. 2014 | Pubmed ID: 25394724 Genetic Validation of a Therapeutic Target in a Mouse Model of ALS Science Translational Medicine. Aug, 2014 | Pubmed ID: 25100738 DNA Methylation Dynamics of the Human Preimplantation Embryo Nature. Jul, 2014 | Pubmed ID: 25079558 FUS is Sequestered in Nuclear Aggregates in ALS Patient Fibroblasts Molecular Biology of the Cell. Sep, 2014 | Pubmed ID: 25009283 Notch Inhibition Allows Oncogene-independent Generation of IPS Cells Nature Chemical Biology. Aug, 2014 | Pubmed ID: 24952596 Ketamine Exposure in Early Development Impairs Specification of the Primary Germ Cell Layers Neurotoxicology and Teratology. May-Jun, 2014 | Pubmed ID: 24746641 Pathways Disrupted in Human ALS Motor Neurons Identified Through Genetic Correction of Mutant SOD1 Cell Stem Cell. Jun, 2014 | Pubmed ID: 24704492 Intrinsic Membrane Hyperexcitability of Amyotrophic Lateral Sclerosis Patient-derived Motor Neurons Cell Reports. Apr, 2014 | Pubmed ID: 24703839 Gene Profiling of Human Induced Pluripotent Stem Cell-derived Astrocyte Progenitors Following Spinal Cord Engraftment Stem Cells Translational Medicine. May, 2014 | Pubmed ID: 24604284 Nanog-independent Reprogramming to IPSCs with Canonical Factors Stem Cell Reports. Feb, 2014 | Pubmed ID: 24527385 Axonal Transport of TDP-43 MRNA Granules is Impaired by ALS-causing Mutations Neuron. Feb, 2014 | Pubmed ID: 24507191 How to Make Spinal Motor Neurons Development (Cambridge, England). Feb, 2014 | Pubmed ID: 24449832 Picking the Lock on Pluripotency The New England Journal of Medicine. Nov, 2013 | Pubmed ID: 24283230 The Mouse C9ORF72 Ortholog is Enriched in Neurons Known to Degenerate in ALS and FTD Nature Neuroscience. Dec, 2013 | Pubmed ID: 24185425 Derivation of Induced Pluripotent Stem Cells from the Baboon: a Nonhuman Primate Model for Preclinical Testing of Stem Cell Therapies Cellular Reprogramming. Dec, 2013 | Pubmed ID: 24182315 Opportunities and Challenges of Pluripotent Stem Cell Neurodegenerative Disease Models Nature Neuroscience. Jul, 2013 | Pubmed ID: 23799470 Modeling Human Disease with Pluripotent Stem Cells: from Genome Association to Function Cell Stem Cell. Jun, 2013 | Pubmed ID: 23746975 Directed Differentiation and Functional Maturation of Cortical Interneurons from Human Embryonic Stem Cells Cell Stem Cell. May, 2013 | Pubmed ID: 23642365 CLP1 Links TRNA Metabolism to Progressive Motor-neuron Loss Nature. Mar, 2013 | Pubmed ID: 23474986 Analysis of Human Embryos from Zygote to Blastocyst Reveals Distinct Gene Expression Patterns Relative to the Mouse Developmental Biology. Mar, 2013 | Pubmed ID: 23261930 Developmental Bias in Cleavage-stage Mouse Blastomeres Current Biology : CB. Jan, 2013 | Pubmed ID: 23177476 SnapShot: Directed Differentiation of Pluripotent Stem Cells Cell. May, 2012 | Pubmed ID: 22632979 Erosion of Dosage Compensation Impacts Human IPSC Disease Modeling Cell Stem Cell. May, 2012 | Pubmed ID: 22560080 Impossibilité De Recrutement De Donneurs De Egg En L'absence D'indemnisation Cell Stem Cell. Oct, 2011 | Pubmed ID: 21982228 Reprogrammation Heures Qui Suivent Le Transfert Nucléaire Dans La Souris, Mais Pas Zygotes Humains Nature Communications. 2011 | Pubmed ID: 21971503 Conversion Des Souris Et Des Fibroblastes Humains En Fonctionnels Motoneurones Spinaux Cell Stem Cell. Sep, 2011 | Pubmed ID: 21852222 Construisant Et Déconstruisant Des Modèles De Cellules Souches De La Maladie Neurologique Neuron. May, 2011 | Pubmed ID: 21609821 Somatique De Codage Mutations Dans L'homme Cellules Souches Pluripotentes Induites Nature. Mar, 2011 | Pubmed ID: 21368825 Cartes De Référence Des ES Humaines Et De La Variation De Cellules IPS Activer Haut Débit Caractérisation Des Lignées Cellulaires Pluripotentes Cell. Feb, 2011 | Pubmed ID: 21295703 Un Ensemble De Test Caractérisé Fonctionnellement De L'homme Cellules Souches Pluripotentes Induites Nature Biotechnology. Mar, 2011 | Pubmed ID: 21293464 Shushing Bas Le Paysage épigénétique à La Différenciation Des Cellules Souches Development (Cambridge, England). Aug, 2010 | Pubmed ID: 20554719 Facteurs Cellule Receveuse Nucléaires Sont Nécessaires Pour La Reprogrammation Par Transfert Nucléaire Development (Cambridge, England). Jun, 2010 | Pubmed ID: 20463036 Sox17 Favorise La Différenciation Dans Les Cellules Souches Embryonnaires De Souris Par Directement Régulation De L'expression Génique Extraembryonnaire Et Indirectement Antagoniser Auto-renouvellement Genes & Development. Feb, 2010 | Pubmed ID: 20123909 Progrès Réalisés En Vue De L'application Clinique Du Patient-spécifiques Les Cellules Souches Pluripotentes The Journal of Clinical Investigation. Jan, 2010 | Pubmed ID: 20051636 Un Inhibiteur De Petite Molécule De Signalisation TGF-bêta Remplace Sox2 Dans La Reprogrammation En Induisant Nanog Cell Stem Cell. Nov, 2009 | Pubmed ID: 19818703 Reprogrammation Après Le Transfert De Chromosomes Dans Les Blastomères De Souris Current Biology : CB. Aug, 2009 | Pubmed ID: 19682906 Numérique ARN Allélotypage Révèle Tissu-spécifique Et Spécifique De L'allèle Expression Des Gènes Dans L'homme Nature Methods. Aug, 2009 | Pubmed ID: 19620972 Séquençage Bisulfite Ciblée Révèle Des Changements Dans La Méthylation De L'ADN Associées à La Reprogrammation Nucléaire Nature Biotechnology. Apr, 2009 | Pubmed ID: 19330000 Le Moment Optimal De L'isolement Cellulaire De Masse Intérieure Augmente L'efficacité De La Dérivation Humain Sur Les Cellules Souches Embryonnaires Et Permet La Génération De Lignées Cellulaires Frères Et Sœurs Cell Stem Cell. Feb, 2009 | Pubmed ID: 19200798 Les Neurones Embryonnaires Humaines Souches Moteur Dérivé Des Cellules Sont Sensibles à L'effet Toxique Des Cellules Gliales Porteuses D'une Mutation De La SLA Qui Causent Cell Stem Cell. Dec, 2008 | Pubmed ID: 19041780 Utilisation Des Cellules Souches Et De La Reprogrammation De Comprendre La Maladie Regenerative Medicine. Nov, 2008 | Pubmed ID: 18947303 Cellules Souches Pluripotentes Induites Générées à Partir De Patients Atteints De SLA Peuvent être Différenciées En Neurones Moteurs Science (New York, N.Y.). Aug, 2008 | Pubmed ID: 18669821 Les Médiateurs De Reprogrammation: Les Facteurs De Transcription Et De Transitions à Travers La Mitose Nature Reviews. Molecular Cell Biology. Jul, 2008 | Pubmed ID: 18568039 Développement épiblaste Court-circuit Cell Stem Cell. Aug, 2007 | Pubmed ID: 18371343 Reprogrammation Du Développement Après Transfert De Chromosomes Dans Les Zygotes De Souris Mitotiques Nature. Jun, 2007 | Pubmed ID: 17554301 Non-cellule à Effet Autonome De Glia Sur Les Neurones Moteurs Dans Une Base De Cellules Souches Embryonnaires Modèle SLA Nature Neuroscience. May, 2007 | Pubmed ID: 17435754 Dolly Legacy: La Transplantation Humaine Nucléaire Et Amélioration Des Médicaments Destinés à Nos Enfants Cloning and Stem Cells. 2007 | Pubmed ID: 17386009 Le Potentiel De La Fusion Des Cellules Pour La Thérapie Humaine Stem Cell Reviews. 2006 | Pubmed ID: 17848721 Une Logique De La Transcription Pour La Reprogrammation Nucléaire Cell. Aug, 2006 | Pubmed ID: 16923385 Les Ovocytes Ovulés Chez La Souris Adulte Dérivez De Non-circulation Cellules Germinales Nature. Jun, 2006 | Pubmed ID: 16799565 La Reprogrammation Nucléaire De Cellules Somatiques Après Fusion Avec Cellules Souches Embryonnaires Humaines Science (New York, N.Y.). Aug, 2005 | Pubmed ID: 16123299 Nucléaire Clonage, La Reprogrammation épigénétique Et La Différenciation Cellulaire Novartis Foundation Symposium. 2005 | Pubmed ID: 16050253 Souris Clonées à Partir De Neurones Sensoriels Olfactifs Nature. Mar, 2004 | Pubmed ID: 14990966 Dérivation De Cellules Germinales Embryonnaires Et Gamètes Mâles à Partir De Cellules Souches Embryonnaires Nature. Jan, 2004 | Pubmed ID: 14668819 Micromanipulating Compensation Du Dosage: Comprendre Inactivation Du Chromosome X Par La Transplantation Nucléaire Seminars in Cell & Developmental Biology. Dec, 2003 | Pubmed ID: 15015742 Différenciation Des F1 Cellules Souches Embryonnaires Dans Viables Souris Mâles Et Femelles Par Complémentation D'embryons Tétraploïdes Methods in Enzymology. 2003 | Pubmed ID: 14696335 Réactivation Incomplète De Oct4 Gènes Liés à Des Embryons De Souris Clonées à Partir Noyaux Somatiques Development (Cambridge, England). Apr, 2003 | Pubmed ID: 12620990 Clonage Nucléaire, Des Cellules Souches, Et Génomique Reprogrammation Cloning and Stem Cells. 2002 | Pubmed ID: 12630413 Expression Du Gène Anormal Chez La Souris Clonées à Partir De Dérivés De Cellules Souches Embryonnaires Et Les Noyaux De Cellules Du Cumulus Proceedings of the National Academy of Sciences of the United States of America. Oct, 2002 | Pubmed ID: 12235366 Souris Mâles Et Femelles Issus De La Même Clone De Cellules Souches Embryonnaires Par Complémentation D'embryons Tétraploïdes Nature Biotechnology. May, 2002 | Pubmed ID: 11981557 Dnmt1 Surexpression Provoque Hyperméthylation Génomique, La Perte D'empreinte, Et Une Létalité Embryonnaire Molecular and Cellular Biology. Apr, 2002 | Pubmed ID: 11884600 Recombinase FLP Réglementée LacZ Expression Au Niveau Du Locus ROSA26 Genesis (New York, N.Y. : 2000). Feb, 2002 | Pubmed ID: 11857816 Modélisation d’infection par le Virus de Zika du développement cerveau humain In Vitro à l’aide de cellules souches dérivées cérébrale organoïdes Max R Salick*1, Michael F Wells*2,3, Kevin Eggan2,3, Ajamete Kaykas1 1Department of Neuroscience, Novartis Institutes for BioMedical Research, 2Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, 3Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University JoVE 56404 Neuroscience Transfert nucléaire dans des ovocytes de la souris Dieter Egli1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 116 Biology Les cellules ES humaines: la culture à partir de cellules congelées Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 86 Biology HUES repiquage souches embryonnaires humaines lignées cellulaires à la trypsine Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 49 Biology La congélation des cellules ES humaines Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 50 Biology
Modélisation d’infection par le Virus de Zika du développement cerveau humain In Vitro à l’aide de cellules souches dérivées cérébrale organoïdes Max R Salick*1, Michael F Wells*2,3, Kevin Eggan2,3, Ajamete Kaykas1 1Department of Neuroscience, Novartis Institutes for BioMedical Research, 2Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, 3Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University JoVE 56404 Neuroscience
Transfert nucléaire dans des ovocytes de la souris Dieter Egli1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 116 Biology
Les cellules ES humaines: la culture à partir de cellules congelées Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 86 Biology
HUES repiquage souches embryonnaires humaines lignées cellulaires à la trypsine Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 49 Biology
La congélation des cellules ES humaines Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 50 Biology