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JoVE Bioengineering Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry JoVE 3559 11/25/2011 Christine M. O'Brien, Kyle Rood, Shramik Sengupta, Sagar K. Gupta, Thiago DeSouza, Aaron Cook, John A. Viator Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology, University of Missouri We have developed a flow cytometer using laser induced ultrasound to detect circulating melanoma cells as an early indicator of metastatic disease.