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Articles by Nada Husic in JoVE

 JoVE Neuroscience

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System


JoVE 4031 5/24/2012

Department of Neurology and Hope Center for Neurological Disorders, Washington University School of Medicine

In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.

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