Petra Schwille Cellular and Molecular Biophysics MPI of Biochemstry Biography Publications Institution JoVE Articles Petra Schwille Petra Schwille is Director at the Max Planck Institute of Biochemistry in Martinsried near Munich, Germany, and Honorary Professor at the LMU Munich. She studied physics and philosophy in Stuttgart and Göttingen, and graduated 1993 with Diploma in Physics at the Georg August University, Göttingen. She obtained her Ph.D. in 1996 from the TU Braunschweig, with a thesis on Fluorescence (Cross-)Correlation Spectroscopy, performed at the MPI for Biophysical Chemistry, Göttingen, with Nobel Laureate Manfred Eigen. After a postdoctoral stay at Cornell University, Ithaca, NY, she returned to the MPI Göttingen as a research group leader, financed by the BMBF Biofuture prize, in 1999. In 2002, she accepted a Chair of Biophysics at the newly established BIOTEC Center of the TU Dresden. Since 2012, she is heading the department Cellular and Molecular Biophysics at the MPI of Biochemistry and Honorary Professor of Physics at LMU Munich. Her scientific interests range from single molecule biophysics to the synthetic biology of reconstituted systems. Publications CTP-controlled Liquid-liquid Phase Separation of ParB Journal of Molecular Biology. 01, 2022 | Pubmed ID: 34902429 Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes The Journal of Physical Chemistry. B. 12, 2021 | Pubmed ID: 34818013 Hidden Protein Functions and What They May Teach Us Trends in Cell Biology. Oct, 2021 | Pubmed ID: 34654605 Mass-sensitive Particle Tracking to Elucidate the Membrane-associated MinDE Reaction Cycle Nature Methods. 10, 2021 | Pubmed ID: 34608318 Actin Crosslinker Competition and Sorting Drive Emergent GUV Size-dependent Actin Network Architecture Communications Biology. 09, 2021 | Pubmed ID: 34584211 Self-organized Protein Patterns: The MinCDE and ParABS Systems Current Opinion in Cell Biology. Oct, 2021 | Pubmed ID: 34399108 Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes Journal of the American Chemical Society. Sep, 2021 | Pubmed ID: 34342967 Tracking Single Particles for Hours Via Continuous DNA-mediated Fluorophore Exchange Nature Communications. 07, 2021 | Pubmed ID: 34290254 Molecular-scale Visualization of Sarcomere Contraction Within Native Cardiomyocytes Nature Communications. 07, 2021 | Pubmed ID: 34215727 Membrane-coated 3D Architectures for Bottom-up Synthetic Biology Soft Matter. Jun, 2021 | Pubmed ID: 34106121 FtsZ Induces Membrane Deformations Via Torsional Stress Upon GTP Hydrolysis Nature Communications. 06, 2021 | Pubmed ID: 34083531 Increasing MinD's Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes ACS Synthetic Biology. 05, 2021 | Pubmed ID: 33881306 Reconstitution of Contractile Actomyosin Rings in Vesicles Nature Communications. 04, 2021 | Pubmed ID: 33859190 De Novo Design of a Reversible Phosphorylation-dependent Switch for Membrane Targeting Nature Communications. 03, 2021 | Pubmed ID: 33674566 Protein Reconstitution Inside Giant Unilamellar Vesicles Annual Review of Biophysics. 05, 2021 | Pubmed ID: 33667121 Probing Biomolecular Interactions by a Pattern-Forming Peptide-Conjugate Sensor Bioconjugate Chemistry. 01, 2021 | Pubmed ID: 33314917 Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles Angewandte Chemie (International Ed. in English). 03, 2021 | Pubmed ID: 33285025 Fine-Tuning Protein Self-Organization by Orthogonal Chemo-Optogenetic Tools Angewandte Chemie (International Ed. in English). 02, 2021 | Pubmed ID: 33155720 Reversible Membrane Deformations by Straight DNA Origami Filaments Soft Matter. Jan, 2021 | Pubmed ID: 32406895 FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps* Angewandte Chemie (International Ed. in English). 11, 2020 | Pubmed ID: 32735732 Phosphoinositides Regulate Force-independent Interactions Between Talin, Vinculin, and Actin ELife. 07, 2020 | Pubmed ID: 32657269 The Speed of FtsZ Treadmilling is Tightly Regulated by Membrane Binding Scientific Reports. 06, 2020 | Pubmed ID: 32591587 Symmetry Breaking and Emergence of Directional Flows in Minimal Actomyosin Cortices Cells. 06, 2020 | Pubmed ID: 32527013 Local Self-Enhancement of MinD Membrane Binding in Min Protein Pattern Formation Journal of Molecular Biology. 05, 2020 | Pubmed ID: 32199984 Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages Small (Weinheim an Der Bergstrasse, Germany). 07, 2020 | Pubmed ID: 32105403 Division in Synthetic Cells Emerging Topics in Life Sciences. Nov, 2019 | Pubmed ID: 33523162 Cell-Free Protein Synthesis and Its Perspectives for Assembling Cells from the Bottom-Up Advanced Biosystems. 06, 2019 | Pubmed ID: 32648712 Cytoskeletal and Actin-Based Polymerization Motors and Their Role in Minimal Cell Design Advanced Biosystems. 06, 2019 | Pubmed ID: 32648711 Design of Biochemical Pattern Forming Systems from Minimal Motifs ELife. 11, 2019 | Pubmed ID: 31767054 An Order of Magnitude Faster DNA-PAINT Imaging by Optimized Sequence Design and Buffer Conditions Nature Methods. 11, 2019 | Pubmed ID: 31591576 Toward Absolute Molecular Numbers in DNA-PAINT Nano Letters. 11, 2019 | Pubmed ID: 31535868 Heated Gas Bubbles Enrich, Crystallize, Dry, Phosphorylate and Encapsulate Prebiotic Molecules Nature Chemistry. 09, 2019 | Pubmed ID: 31358919 Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System Chembiochem : a European Journal of Chemical Biology. 10, 2019 | Pubmed ID: 31344304 The E. Coli MinCDE System in the Regulation of Protein Patterns and Gradients Cellular and Molecular Life Sciences : CMLS. Nov, 2019 | Pubmed ID: 31317204 Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes Methods in Molecular Biology (Clifton, N.J.). Month, 2019 | Pubmed ID: 31218628 Bottom-up Synthetic Biology: Reconstitution in Space and Time Current Opinion in Biotechnology. 12, 2019 | Pubmed ID: 31176995 Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer ACS Applied Materials & Interfaces. Jun, 2019 | Pubmed ID: 31136146 Synthetic Cell Division Via Membrane-transforming Molecular Assemblies BMC Biology. 05, 2019 | Pubmed ID: 31126285 Manfred Eigen (1927-2019) Angewandte Chemie (International Ed. in English). Jul, 2019 | Pubmed ID: 31124258 Temperature-sensitive Protein Expression in Protocells Chemical Communications (Cambridge, England). May, 2019 | Pubmed ID: 31094370 Flat-top TIRF Illumination Boosts DNA-PAINT Imaging and Quantification Nature Communications. 03, 2019 | Pubmed ID: 30894522 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics Nano Letters. 04, 2019 | Pubmed ID: 30864449 Optical Manipulation of Sphingolipid Biosynthesis Using Photoswitchable Ceramides ELife. 02, 2019 | Pubmed ID: 30720434 More from Less - Bottom-up Reconstitution of Cell Biology Journal of Cell Science. 02, 2019 | Pubmed ID: 30718262 Single Particle Tracking and Super-Resolution Imaging of Membrane-Assisted Stop-and-Go Diffusion and Lattice Assembly of DNA Origami ACS Nano. 02, 2019 | Pubmed ID: 30588792 In Vitro Reconstitution of the Bacterial Cytoskeleton: Expected and Unexpected New Insights Microbial Biotechnology. 01, 2019 | Pubmed ID: 30411506 Myosin-II Activity Generates a Dynamic Steady State with Continuous Actin Turnover in a Minimal Actin Cortex Journal of Cell Science. 12, 2018 | Pubmed ID: 30538127 Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy Molecules (Basel, Switzerland). Nov, 2018 | Pubmed ID: 30513691 Plasmonic Nanosensors Reveal a Height Dependence of MinDE Protein Oscillations on Membrane Features Journal of the American Chemical Society. 12, 2018 | Pubmed ID: 30481454 Membrane Association and Remodeling by Intraflagellar Transport Protein IFT172 Nature Communications. 11, 2018 | Pubmed ID: 30409972 Light-Induced Printing of Protein Structures on Membranes in Vitro Nano Letters. 11, 2018 | Pubmed ID: 30295028 Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations Angewandte Chemie (International Ed. in English). 12, 2018 | Pubmed ID: 30270475 The MinDE System is a Generic Spatial Cue for Membrane Protein Distribution in Vitro Nature Communications. 09, 2018 | Pubmed ID: 30258191 Control of Membrane Binding and Diffusion of Cholesteryl-Modified DNA Origami Nanostructures by DNA Spacers Langmuir : the ACS Journal of Surfaces and Colloids. 12, 2018 | Pubmed ID: 30253101 Liposomes and Polymersomes: a Comparative Review Towards Cell Mimicking Chemical Society Reviews. Sep, 2018 | Pubmed ID: 30177983 MaxSynBio - Avenues Towards Creating Cells from the Bottom Up Angewandte Chemie (International Ed. in English). May, 2018 | Pubmed ID: 29749673 Photophysical Behavior of MNeonGreen, an Evolutionarily Distant Green Fluorescent Protein Biophysical Journal. May, 2018 | Pubmed ID: 29706225 MinE Conformational Switching Confers Robustness on Self-organized Min Protein Patterns Proceedings of the National Academy of Sciences of the United States of America. May, 2018 | Pubmed ID: 29666276 Quantifying Reversible Surface Binding Via Surface-Integrated Fluorescence Correlation Spectroscopy Nano Letters. May, 2018 | Pubmed ID: 29658275 Reverse and Forward Engineering of Protein Pattern Formation Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. May, 2018 | Pubmed ID: 29632258 FCS Analysis of Protein Mobility on Lipid Monolayers Biophysical Journal. May, 2018 | Pubmed ID: 29605081 There and Back Again: from the Origin of Life to Single Molecules European Biophysics Journal : EBJ. May, 2018 | Pubmed ID: 29569181 Membrane Sculpting by Curved DNA Origami Scaffolds Nature Communications. 02, 2018 | Pubmed ID: 29476101 Optical Control of a Biological Reaction-Diffusion System Angewandte Chemie (International Ed. in English). Feb, 2018 | Pubmed ID: 29266672 High-Speed Atomic Force Microscopy Reveals the Inner Workings of the MinDE Protein Oscillator Nano Letters. Jan, 2018 | Pubmed ID: 29210266 Biology and the Art of Abstraction Biophysical Reviews. Aug, 2017 | Pubmed ID: 28756511 Cell-free Protein Synthesis in Micro Compartments: Building a Minimal Cell from Biobricks New Biotechnology. Oct, 2017 | Pubmed ID: 28690157 Revolving Around Constriction by ESCRT-III Nature Cell Biology. Jun, 2017 | Pubmed ID: 28659638 Large-scale Modulation of Reconstituted Min Protein Patterns and Gradients by Defined Mutations in MinE's Membrane Targeting Sequence PloS One. Month, 2017 | Pubmed ID: 28622374 Reconstitution of Protein Dynamics Involved in Bacterial Cell Division Sub-cellular Biochemistry. Month, 2017 | Pubmed ID: 28500535 Control of Lipid Domain Organization by a Biomimetic Contractile Actomyosin Cortex ELife. 05, 2017 | Pubmed ID: 28463108 Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes The Journal of Membrane Biology. Aug, 2017 | Pubmed ID: 27826635 Transport Efficiency of Membrane-anchored Kinesin-1 Motors Depends on Motor Density and Diffusivity Proceedings of the National Academy of Sciences of the United States of America. 11, 2016 | Pubmed ID: 27803325 In Vitro Reconstitution of a Membrane Switch Mechanism for the Polarity Protein LGL Journal of Molecular Biology. 12, 2016 | Pubmed ID: 27720986 Optical Control of Lipid Rafts with Photoswitchable Ceramides Journal of the American Chemical Society. 10, 2016 | Pubmed ID: 27626130 Coordinated Recruitment of Spir Actin Nucleators and Myosin V Motors to Rab11 Vesicle Membranes ELife. 09, 2016 | Pubmed ID: 27623148 Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets Angewandte Chemie (International Ed. in English). Oct, 2016 | Pubmed ID: 27465495 Single Particle Plasmon Sensors As Label-Free Technique To Monitor MinDE Protein Wave Propagation on Membranes Nano Letters. Jun, 2016 | Pubmed ID: 27172130 DNA Nanostructures on Membranes As Tools for Synthetic Biology Biophysical Journal. 04, 2016 | Pubmed ID: 27119630 Pattern Formation on Membranes and Its Role in Bacterial Cell Division Current Opinion in Cell Biology. Feb, 2016 | Pubmed ID: 26915065 Diffusion Coefficients and Dissociation Constants of Enhanced Green Fluorescent Protein Binding to Free Standing Membranes Data in Brief. Dec, 2015 | Pubmed ID: 26587560 Introducing a Fluorescence-based Standard to Quantify Protein Partitioning into Membranes Biochimica Et Biophysica Acta. Nov, 2015 | Pubmed ID: 26342678 Jump-starting Life? Fundamental Aspects of Synthetic Biology The Journal of Cell Biology. Aug, 2015 | Pubmed ID: 26323686 FtsZ Polymers Tethered to the Membrane by ZipA Are Susceptible to Spatial Regulation by Min Waves Biophysical Journal. May, 2015 | Pubmed ID: 25954894 Adaptive Lipid Packing and Bioactivity in Membrane Domains PloS One. Month, 2015 | Pubmed ID: 25905447 Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles Angewandte Chemie (International Ed. in English). May, 2015 | Pubmed ID: 25882792 Cytoskeletal Pinning Controls Phase Separation in Multicomponent Lipid Membranes Biophysical Journal. Mar, 2015 | Pubmed ID: 25762322 Toward Spatially Regulated Division of Protocells: Insights into the E. Coli Min System from in Vitro Studies Life (Basel, Switzerland). Dec, 2014 | Pubmed ID: 25513760 Reconstitution of Self-organizing Protein Gradients As Spatial Cues in Cell-free Systems ELife. Oct, 2014 | Pubmed ID: 25271375 Fluorescence Fluctuation Microscopy: a Diversified Arsenal of Methods to Investigate Molecular Dynamics Inside Cells Current Opinion in Structural Biology. Oct, 2014 | Pubmed ID: 25126766 Surface Topology Assisted Alignment of Min Protein Waves FEBS Letters. Aug, 2014 | Pubmed ID: 24937143 ESCRT-III Mediated Cell Division in Sulfolobus Acidocaldarius - a Reconstitution Perspective Frontiers in Microbiology. Month, 2014 | Pubmed ID: 24926288 Asymmetric Supported Lipid Bilayer Formation Via Methyl-β-cyclodextrin Mediated Lipid Exchange: Influence of Asymmetry on Lipid Dynamics and Phase Behavior Langmuir : the ACS Journal of Surfaces and Colloids. Jul, 2014 | Pubmed ID: 24885372 MinCDE Exploits the Dynamic Nature of FtsZ Filaments for Its Spatial Regulation Proceedings of the National Academy of Sciences of the United States of America. Apr, 2014 | Pubmed ID: 24707052 Bacterial Cell Division: a Swirling Ring to Rule Them All? Current Biology : CB. Feb, 2014 | Pubmed ID: 24556438 MinC, MinD, and MinE Drive Counter-oscillation of Early-cell-division Proteins Prior to Escherichia Coli Septum Formation MBio. Dec, 2013 | Pubmed ID: 24327341 Propagation of MinCDE Waves on Free-standing Membranes Environmental Microbiology. Dec, 2013 | Pubmed ID: 24118679 The Design of MACs (minimal Actin Cortices) Cytoskeleton (Hoboken, N.J.). Nov, 2013 | Pubmed ID: 24039068 Lateral Membrane Diffusion Modulated by a Minimal Actin Cortex Biophysical Journal. Apr, 2013 | Pubmed ID: 23561523 Photoconversion of Bodipy-labeled Lipid Analogues Chembiochem : a European Journal of Chemical Biology. Apr, 2013 | Pubmed ID: 23512865 Myosin Motors Fragment and Compact Membrane-bound Actin Filaments ELife. Jan, 2013 | Pubmed ID: 23326639 Reconstitution of Pole-to-pole Oscillations of Min Proteins in Microengineered Polydimethylsiloxane Compartments Angewandte Chemie (International Ed. in English). Jan, 2013 | Pubmed ID: 23184489 Towards a Bottom-up Reconstitution of Bacterial Cell Division Trends in Cell Biology. Dec, 2012 | Pubmed ID: 23067680 Minimal Systems to Study Membrane-cytoskeleton Interactions Current Opinion in Biotechnology. Oct, 2012 | Pubmed ID: 22503237 Importin 8 is a Gene Silencing Factor That Targets Argonaute Proteins to Distinct MRNAs Cell. Feb, 2009 | Pubmed ID: 19167051 Fluorescence Correlation Spectroscopy and Fluorescence Cross-correlation Spectroscopy Reveal the Cytoplasmic Origination of Loaded Nuclear RISC in Vivo in Human Cells Nucleic Acids Research. Nov, 2008 | Pubmed ID: 18842624 Massagevoelige deeltjestracking om membraangeassocieerde macromolecuuldynamica te karakteriseren Frederik Steiert1,2, Tamara Heermann1, Nikolas Hundt3, Petra Schwille1 1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 2Department of Physics, Technical University Munich, 3Department of Cellular Physiology, Biomedical Center (BMC), Ludwig-Maximilians-Universität München JoVE 63583 Biochemistry Snelle inkapseling van gereconstitueerd cytoskelet in gigantische unilamellaire blaasjes Yashar Bashirzadeh*1, Nadab Wubshet*1, Thomas Litschel2, Petra Schwille3, Allen P. Liu1,4,5,6 1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor JoVE 63332 Bioengineering In Vitro Reconstructie van het zelf-organiserende eiwit patronen op ondersteunde lipide Bilayers Beatrice Ramm*1, Philipp Glock*1, Petra Schwille1 1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry JoVE 58139 Biochemistry
Massagevoelige deeltjestracking om membraangeassocieerde macromolecuuldynamica te karakteriseren Frederik Steiert1,2, Tamara Heermann1, Nikolas Hundt3, Petra Schwille1 1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 2Department of Physics, Technical University Munich, 3Department of Cellular Physiology, Biomedical Center (BMC), Ludwig-Maximilians-Universität München JoVE 63583 Biochemistry
Snelle inkapseling van gereconstitueerd cytoskelet in gigantische unilamellaire blaasjes Yashar Bashirzadeh*1, Nadab Wubshet*1, Thomas Litschel2, Petra Schwille3, Allen P. Liu1,4,5,6 1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor JoVE 63332 Bioengineering
In Vitro Reconstructie van het zelf-organiserende eiwit patronen op ondersteunde lipide Bilayers Beatrice Ramm*1, Philipp Glock*1, Petra Schwille1 1Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry JoVE 58139 Biochemistry