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Journal / Bioengineering / Snelle inkapseling van gereconstitueerd…
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Bioengineering

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JoVE Journal Bioengineering
Snelle inkapseling van gereconstitueerd cytoskelet in gigantische unilamellaire blaasjes
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Snelle inkapseling van gereconstitueerd cytoskelet in gigantische unilamellaire blaasjes

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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Dit artikel introduceert een eenvoudige methode voor snelle productie van gigantische unilamellaire blaasjes met ingekapselde cytoskeletale eiwitten. De methode blijkt nuttig te zijn voor bottom-up reconstitutie van cytoskeletstructuren in opsluiting en cytoskelet-membraaninteracties.

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Bio-engineering Emulsie transfer cDICE GUVs bottom-up reconstitutie actine fascin
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