Robert David Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC) Rostock University Medical Center Biography Publications Institution JoVE Articles Robert David has not added a biography. If you are Robert David and would like to personalize this page please email our Author Liaison for assistance. Publications Hematopoietic Stem-cell Senescence and Myocardial Repair - Coronary Artery Disease Genotype/phenotype Analysis of Post-MI Myocardial Regeneration Response Induced by CABG/CD133+ Bone Marrow Hematopoietic Stem Cell Treatment in RCT PERFECT Phase 3 EBioMedicine. Jul, 2020 | Pubmed ID: 32629392 Dose-Independent Therapeutic Benefit of Bone Marrow Stem Cell Transplantation After MI in Mice Biomedicines. Jun, 2020 | Pubmed ID: 32545336 [Ga]-NODAGA-RGD Positron Emission Tomography (PET) for Assessment of Post Myocardial Infarction Angiogenesis As a Predictor for Left Ventricular Remodeling in Mice After Cardiac Stem Cell Therapy Cells. May, 2020 | Pubmed ID: 32486211 F-FDG PET-Based Imaging of Myocardial Inflammation Following Acute Myocardial Infarction in a Mouse Model International Journal of Molecular Sciences. May, 2020 | Pubmed ID: 32397287 Integrative Cluster Analysis of Whole Hearts Reveals Proliferative Cardiomyocytes in Adult Mice Cells. May, 2020 | Pubmed ID: 32384695 Quantitative Evaluation of the Sarcomere Network of Human HiPSC-Derived Cardiomyocytes Using Single-Molecule Localization Microscopy International Journal of Molecular Sciences. Apr, 2020 | Pubmed ID: 32316650 GLP: A Requirement in Cell Therapies - Perspectives for the Cardiovascular Field Advanced Drug Delivery Reviews. Apr, 2020 | Pubmed ID: 32305352 Single Nuclei Sequencing of Entire Mammalian Hearts: Strain-dependent Cell-type Composition and Velocity Cardiovascular Research. Jun, 2020 | Pubmed ID: 32243511 Cardioprotective Potential of Human Endothelial-Colony Forming Cells from Diabetic and Nondiabetic Donors Cells. 03, 2020 | Pubmed ID: 32131432 SNX27 Regulates DRA Activity and Mediates Its Direct Recycling by PDZ-interaction in Early Endosomes at the Apical Pole of Caco2 Cells American Journal of Physiology. Gastrointestinal and Liver Physiology. 05, 2020 | Pubmed ID: 32116023 RNA-Based Strategies for Cardiac Reprogramming of Human Mesenchymal Stromal Cells Cells. 02, 2020 | Pubmed ID: 32098400 Single-Nucleus Sequencing of an Entire Mammalian Heart: Cell Type Composition and Velocity Cells. 01, 2020 | Pubmed ID: 32013057 Angiogenic Potential of Bone Marrow Derived CD133 and CD271 Intramyocardial Stem Cell Trans- Plantation Post MI Cells. Dec, 2019 | Pubmed ID: 31892273 18F-FDG PET-Based Imaging of Myocardial Inflammation Predicts a Functional Outcome Following Transplantation of MESC-Derived Cardiac Induced Cells in a Mouse Model of Myocardial Infarction Cells. 12, 2019 | Pubmed ID: 31835854 CD271 Human Mesenchymal Stem Cells Show Antiarrhythmic Effects in a Novel Murine Infarction Model Cells. 11, 2019 | Pubmed ID: 31757119 HiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us Cells. 10, 2019 | Pubmed ID: 31661896 Nkx2.5 Based Ventricular Programming of Murine ESC-Derived Cardiomyocytes Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2019 | Pubmed ID: 31373783 Vimentin-Induced Cardiac Mesenchymal Stem Cells Proliferate in the Acute Ischemic Myocardium Cells, Tissues, Organs. 2018 | Pubmed ID: 30630170 Stem Cell Therapy in Heart Diseases - Cell Types, Mechanisms and Improvement Strategies Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2018 | Pubmed ID: 30121644 Current IGFBP-Related Biomarker Research in Cardiovascular Disease-We Need More Structural and Functional Information in Clinical Studies Frontiers in Endocrinology. 2018 | Pubmed ID: 30061864 Intramyocardial Angiogenetic Stem Cells and Epicardial Erythropoietin Save the Acute Ischemic Heart Disease Models & Mechanisms. May, 2018 | Pubmed ID: 29752300 Specific Cell (Re-)Programming: Approaches and Perspectives Advances in Biochemical Engineering/biotechnology. 2018 | Pubmed ID: 29071403 Cardiac Mesenchymal Stem Cells Proliferate Early in the Ischemic Heart European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes. 2017 | Pubmed ID: 29073604 (Re-)programming of Subtype Specific Cardiomyocytes Advanced Drug Delivery Reviews. Oct, 2017 | Pubmed ID: 28916499 Mechanisms of Stem Cell Based Cardiac Repair-gap Junctional Signaling Promotes the Cardiac Lineage Specification of Mesenchymal Stem Cells Scientific Reports. Aug, 2017 | Pubmed ID: 28852100 Cardiac Function Improvement and Bone Marrow Response -: Outcome Analysis of the Randomized PERFECT Phase III Clinical Trial of Intramyocardial CD133(+) Application After Myocardial Infarction EBioMedicine. Aug, 2017 | Pubmed ID: 28781130 Data on the Fate of MACS® MicroBeads Intramyocardially Co-injected with Stem Cell Products Data in Brief. Aug, 2017 | Pubmed ID: 28706966 Cardiac Cell Therapies for the Treatment of Acute Myocardial Infarction: A Meta-Analysis from Mouse Studies Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. May, 2017 | Pubmed ID: 28535507 Intramyocardial Fate and Effect of Iron Nanoparticles Co-injected with MACS(®) Purified Stem Cell Products Biomaterials. Aug, 2017 | Pubmed ID: 28494265 Thymosin β4 Improves Differentiation and Vascularization of EHTs Stem Cells International. 2017 | Pubmed ID: 28191018 GMP-conformant On-site Manufacturing of a CD133(+) Stem Cell Product for Cardiovascular Regeneration Stem Cell Research & Therapy. Feb, 2017 | Pubmed ID: 28187777 Magnet-Bead Based MicroRNA Delivery System to Modify CD133(+) Stem Cells Stem Cells International. 2016 | Pubmed ID: 27795713 Applying 3D-FRAP Microscopy to Analyse Gap Junction-dependent Shuttling of Small Antisense RNAs Between Cardiomyocytes Journal of Molecular and Cellular Cardiology. Sep, 2016 | Pubmed ID: 27480520 Non-viral Magnetic Engineering of Endothelial Cells with MicroRNA and Plasmid-DNA-An Optimized Targeting Approach Nanomedicine : Nanotechnology, Biology, and Medicine. Nov, 2016 | Pubmed ID: 27389150 TRAPLINE: a Standardized and Automated Pipeline for RNA Sequencing Data Analysis, Evaluation and Annotation BMC Bioinformatics. Jan, 2016 | Pubmed ID: 26738481 Cardiac FDG-PET: a Straight Forward Tool with High Potential European Heart Journal Cardiovascular Imaging. Feb, 2016 | Pubmed ID: 26510929 Gap Junctional Shuttling of MiRNA--A Novel Pathway of Intercellular Gene Regulation and Its Prospects in Clinical Application Cellular Signalling. Dec, 2015 | Pubmed ID: 26391653 Exploiting AT2R to Improve CD117 Stem Cell Function In Vitro and In Vivo--Perspectives for Cardiac Stem Cell Therapy Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2015 | Pubmed ID: 26303045 The CD4(+) AT2R(+) T Cell Subpopulation Improves Post-infarction Remodelling and Restores Cardiac Function Journal of Cellular and Molecular Medicine. Aug, 2015 | Pubmed ID: 25991381 (124)I-PET Assessment of Human Sodium Iodide Symporter Reporter Gene Activity for Highly Sensitive In Vivo Monitoring of Teratoma Formation in Mice Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging. Dec, 2015 | Pubmed ID: 25896817 A 3'-UTR Mutation Creates a MicroRNA Target Site in the GFPT1 Gene of Patients with Congenital Myasthenic Syndrome Human Molecular Genetics. Jun, 2015 | Pubmed ID: 25765662 Defining Optimized Properties of Modified MRNA to Enhance Virus- and DNA- Independent Protein Expression in Adult Stem Cells and Fibroblasts Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2015 | Pubmed ID: 25720503 Coevolution in Management Fashion: an Agent-based Model of Consultant-driven Innovation AJS; American Journal of Sociology. Jul, 2014 | Pubmed ID: 25705784 Selective Migration of Subpopulations of Bone Marrow Cells Along an SDF-1α and ATP Gradient Bone Marrow Research. 2014 | Pubmed ID: 25610653 Tissue Cohesion and the Mechanics of Cell Rearrangement Development (Cambridge, England). Oct, 2014 | Pubmed ID: 25249459 A Theory for the Surface Tensions and Contact Angles of Hydrogen-bonding Liquids Langmuir : the ACS Journal of Surfaces and Colloids. Oct, 2014 | Pubmed ID: 25203680 In-vivo Comparison of the Acute Retention of Stem Cell Derivatives and Fibroblasts After Intramyocardial Transplantation in the Mouse Model European Journal of Nuclear Medicine and Molecular Imaging. Dec, 2014 | Pubmed ID: 25063040 Increased Numbers of Bone Marrow-derived Cells in Parathyroid Adenoma European Journal of Clinical Investigation. Sep, 2014 | Pubmed ID: 25047264 Programming and Isolation of Highly Pure Physiologically and Pharmacologically Functional Sinus-nodal Bodies from Pluripotent Stem Cells Stem Cell Reports. May, 2014 | Pubmed ID: 24936448 Impact of Short-term Liquid Storage on Human CD133(+) Stem Cells Cell Transplantation. May, 2014 | Pubmed ID: 24800805 Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105(+) HMSCs Stem Cells International. 2014 | Pubmed ID: 24799915 Control of IGFBP-2 Expression by Steroids and Peptide Hormones in Vertebrates Frontiers in Endocrinology. 2014 | Pubmed ID: 24778626 Early Pregnancy Urinary Biomarkers of Fatty Acid and Carbohydrate Metabolism in Pregnancies Complicated by Gestational Diabetes Diabetes Research and Clinical Practice. Jun, 2014 | Pubmed ID: 24703806 Contact Angle Patterns on Low-energy Surfaces Advances in Colloid and Interface Science. Apr, 2014 | Pubmed ID: 23601233 Human Mesenchymal Stem Cells Display Reduced Expression of CD105 After Culture in Serum-Free Medium Stem Cells International. 2013 | Pubmed ID: 24194767 Enhanced Stem Cell Migration Mediated by VCAM-1/VLA-4 Interaction Improves Cardiac Function in Virus-induced Dilated Cardiomyopathy Basic Research in Cardiology. Nov, 2013 | Pubmed ID: 24065117 Positron Emission Tomography Based In-vivo Imaging of Early Phase Stem Cell Retention After Intramyocardial Delivery in the Mouse Model European Journal of Nuclear Medicine and Molecular Imaging. Oct, 2013 | Pubmed ID: 23860738 Innovative Strategy for MicroRNA Delivery in Human Mesenchymal Stem Cells Via Magnetic Nanoparticles International Journal of Molecular Sciences. 2013 | Pubmed ID: 23702843 Antidiabetic Gliptins in Combination with G-CSF Enhances Myocardial Function and Survival After Acute Myocardial Infarction International Journal of Cardiology. Oct, 2013 | Pubmed ID: 23669105 Reconsidering Pluripotency Tests: Do We Still Need Teratoma Assays? Stem Cell Research. Jul, 2013 | Pubmed ID: 23611953 Contact Angle Hysteresis on Randomly Rough Surfaces: a Computational Study Langmuir : the ACS Journal of Surfaces and Colloids. Apr, 2013 | Pubmed ID: 23506209 Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-associated Oct-25 Gene PLoS Genetics. 2013 | Pubmed ID: 23382689 Cardiac Differentiation in Xenopus is Initiated by Mespa Cardiovascular Research. Mar, 2013 | Pubmed ID: 23241315 Selection of a Common Multipotent Cardiovascular Stem Cell Using the 3.4-kb MesP1 Promoter Fragment Basic Research in Cardiology. Jan, 2013 | Pubmed ID: 23184392 Directing Cardiomyogenic Differentiation of Human Pluripotent Stem Cells by Plasmid-based Transient Overexpression of Cardiac Transcription Factors Stem Cells and Development. Apr, 2013 | Pubmed ID: 23157212 Audit of Clinical-laboratory Practices in Haematology and Blood Transfusion at Muhimbili National Hospital in Tanzania Tanzania Journal of Health Research. Oct, 2012 | Pubmed ID: 26591723 From Pluripotency to Distinct Cardiomyocyte Subtypes Physiology (Bethesda, Md.). Jun, 2012 | Pubmed ID: 22689787 OxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 Mediators of Inflammation. 2012 | Pubmed ID: 22619482 Cadherin-dependent Differential Cell Adhesion in Xenopus Causes Cell Sorting in Vitro but Not in the Embryo Journal of Cell Science. Apr, 2012 | Pubmed ID: 22328523 Safety and Effect on Rod Function of ACU-4429, a Novel Small-molecule Visual Cycle Modulator Retina (Philadelphia, Pa.). Jan, 2012 | Pubmed ID: 21519291 Mitochondrial Thioredoxin Reductase is Essential for Early Postischemic Myocardial Protection Circulation. Dec, 2011 | Pubmed ID: 22144571 Stage-specific Histone Modification Profiles Reveal Global Transitions in the Xenopus Embryonic Epigenome PloS One. 2011 | Pubmed ID: 21814581 Induction of MesP1 by Brachyury(T) Generates the Common Multipotent Cardiovascular Stem Cell Cardiovascular Research. Oct, 2011 | Pubmed ID: 21632880 Large-scale Mechanical Properties of Xenopus Embryonic Epithelium Proceedings of the National Academy of Sciences of the United States of America. Mar, 2011 | Pubmed ID: 21368110 Parathyroid Hormone is a DPP-IV Inhibitor and Increases SDF-1-driven Homing of CXCR4(+) Stem Cells into the Ischaemic Heart Cardiovascular Research. Jun, 2011 | Pubmed ID: 21245057 Intraocular Pressure Changes in the Contralateral Eye After Topical Treatment: Does an "ophthalmotonic Consensual Reaction" Exist? The Israel Medical Association Journal : IMAJ. Sep, 2010 | Pubmed ID: 21287803 The Louisiana Physician As Witness The Journal of the Louisiana State Medical Society : Official Organ of the Louisiana State Medical Society. Jul-Aug, 2010 | Pubmed ID: 20882818 Computation of Contact Lines on Randomly Heterogeneous Surfaces Langmuir : the ACS Journal of Surfaces and Colloids. Aug, 2010 | Pubmed ID: 20695567 Assessment of Human MAPCs for Stem Cell Transplantation and Cardiac Regeneration After Myocardial Infarction in SCID Mice Experimental Hematology. Nov, 2010 | Pubmed ID: 20621157 Do Omega-6 and Trans Fatty Acids Play a Role in Complex Regional Pain Syndrome? A Pilot Study Pain Medicine (Malden, Mass.). Jul, 2010 | Pubmed ID: 20545870 Forward Programming of Pluripotent Stem Cells Towards Distinct Cardiovascular Cell Types Cardiovascular Research. Nov, 2009 | Pubmed ID: 19564151 Microencapsulated Foods As a Functional Delivery Vehicle for Omega-3 Fatty Acids: a Pilot Study Journal of the International Society of Sports Nutrition. 2009 | Pubmed ID: 19480650 Tissue Surface Tension Measurement by Rigorous Axisymmetric Drop Shape Analysis Colloids and Surfaces. B, Biointerfaces. Sep, 2009 | Pubmed ID: 19442498 Synergy Between CD26/DPP-IV Inhibition and G-CSF Improves Cardiac Function After Acute Myocardial Infarction Cell Stem Cell. Apr, 2009 | Pubmed ID: 19341621 Axisymmetric Drop Shape Analysis for Estimating the Surface Tension of Cell Aggregates by Centrifugation Biophysical Journal. Feb, 2009 | Pubmed ID: 19217876 Erythropoietin Administration After Myocardial Infarction in Mice Attenuates Ischemic Cardiomyopathy Associated with Enhanced Homing of Bone Marrow-derived Progenitor Cells Via the CXCR-4/SDF-1 Axis FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Feb, 2009 | Pubmed ID: 18827024 Maternal Plasma 25-hydroxyvitamin D Concentrations and the Risk for Gestational Diabetes Mellitus PloS One. 2008 | Pubmed ID: 19015731 Endogenous Nitric Oxide Synthase Inhibitors, Arterial Hemodynamics, and Subclinical Vascular Disease: the PREVENCION Study Hypertension. Dec, 2008 | Pubmed ID: 18852383 Provisional Restoration for an Osseointegrated Single Maxillary Anterior Implant Journal (Canadian Dental Association). Sep, 2008 | Pubmed ID: 18789193 Parathyroid Hormone Effectively Induces Mobilization of Progenitor Cells Without Depletion of Bone Marrow Experimental Hematology. Sep, 2008 | Pubmed ID: 18504066 Mapping and Sequencing of Structural Variation from Eight Human Genomes Nature. May, 2008 | Pubmed ID: 18451855 G-CSF Treatment After Myocardial Infarction: Impact on Bone Marrow-derived Vs Cardiac Progenitor Cells Experimental Hematology. Jun, 2008 | Pubmed ID: 18346841 Parathyroid Hormone Treatment After Myocardial Infarction Promotes Cardiac Repair by Enhanced Neovascularization and Cell Survival Cardiovascular Research. Mar, 2008 | Pubmed ID: 18055578 Evolution of Genes and Genomes on the Drosophila Phylogeny Nature. Nov, 2007 | Pubmed ID: 17994087 Empirical Equation to Account for the Length Dependence of Line Tension Langmuir : the ACS Journal of Surfaces and Colloids. Nov, 2007 | Pubmed ID: 17949026 Circulation of CD34+ Progenitor Cell Populations in Patients with Idiopathic Dilated and Ischaemic Cardiomyopathy (DCM and ICM) European Heart Journal. May, 2007 | Pubmed ID: 17395679 A Prospective Study of Early-pregnancy Plasma Malondialdehyde Concentration and Risk of Preeclampsia Clinical Biochemistry. Jul, 2006 | Pubmed ID: 16603146 The Finished DNA Sequence of Human Chromosome 12 Nature. Mar, 2006 | Pubmed ID: 16541075 Erythrocyte Omega-3 and Omega-6 Polyunsaturated Fatty Acids and Preeclampsia Risk in Peruvian Women Archives of Gynecology and Obstetrics. May, 2006 | Pubmed ID: 16520922 Prophylaxis for Second Eye Involvement in Leber Hereditary Optic Neuropathy: an Open-labeled, Nonrandomized Multicenter Trial of Topical Brimonidine Purite American Journal of Ophthalmology. Sep, 2005 | Pubmed ID: 16083844 Magnetic Cell Sorting Purification of Differentiated Embryonic Stem Cells Stably Expressing Truncated Human CD4 As Surface Marker Stem Cells (Dayton, Ohio). Apr, 2005 | Pubmed ID: 15790768 The DNA Sequence of the Human X Chromosome Nature. Mar, 2005 | Pubmed ID: 15772651 Minimizing the Dark Air Medical Journal. Nov-Dec, 2003 | Pubmed ID: 14762990 Stem-cell Homing and Tissue Regeneration in Ischaemic Cardiomyopathy Lancet. Aug, 2003 | Pubmed ID: 12957085 Human Embryonic Stem Cells for Cardiovascular Repair Cardiovascular Research. May, 2003 | Pubmed ID: 12757866 Recognition of Adenosine Triphosphate Binding Sites Using Parallel Cascade System Identification Annals of Biomedical Engineering. Apr, 2003 | Pubmed ID: 12723687 A SIN Lentiviral Vector Containing PIGA CDNA Allows Long-term Phenotypic Correction of CD34+-derived Cells from Patients with Paroxysmal Nocturnal Hemoglobinuria Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2003 | Pubmed ID: 12668126 Pathogen Discovery from Human Tissue by Sequence-based Computational Subtraction Genomics. Mar, 2003 | Pubmed ID: 12659816 Initial Sequencing and Comparative Analysis of the Mouse Genome Nature. Dec, 2002 | Pubmed ID: 12466850 Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy Lisa Johann1,2, Oleksandra Chabanovska1,2, Cajetan Immanuel Lang3, Robert David1,2, Heiko Lemcke1,2 1Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Rostock University Medical Center, 2Faculty of Interdisciplinary Research, Department Life, Light & Matter, University Rostock, 3Department of Cardiology, Rostock University Medical Center JoVE 61605 Biology Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells Paula Müller*1,2, Katharina Ekat*3, Anne Brosemann3, Anne Köntges3, Robert David1,2, Hermann Lang3 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology, Rostock University Medical Center JoVE 58089 Genetics Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting Frauke Hausburg*1,2, Paula Müller*1,2, Natalia Voronina*1, Gustav Steinhoff1,2, Robert David1,2 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University JoVE 57474 Bioengineering Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy Heiko Lemcke1,2,3, Natalia Voronina1,2,3, Gustav Steinhoff1,2,3, Robert David1,2,3 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery, University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock JoVE 55870 Biology Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells Natalia Voronina1, Heiko Lemcke1, Frank Wiekhorst2, Jens-Peter Kühn3, Markus Frank4, Gustav Steinhoff1, Robert David1 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, 2Physikalisch-Technische Bundesanstalt, 3Department of Radiology and Neuroradiology, Ernst-Moritz-Arndt-University Greifswald, 4Electron Microscopy Center, University of Rostock JoVE 55567 Medicine Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection Christian Rimmbach1, Julia J. Jung1, Robert David1 1Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock JoVE 52465 Developmental Biology
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy Lisa Johann1,2, Oleksandra Chabanovska1,2, Cajetan Immanuel Lang3, Robert David1,2, Heiko Lemcke1,2 1Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Rostock University Medical Center, 2Faculty of Interdisciplinary Research, Department Life, Light & Matter, University Rostock, 3Department of Cardiology, Rostock University Medical Center JoVE 61605 Biology
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells Paula Müller*1,2, Katharina Ekat*3, Anne Brosemann3, Anne Köntges3, Robert David1,2, Hermann Lang3 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology, Rostock University Medical Center JoVE 58089 Genetics
Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting Frauke Hausburg*1,2, Paula Müller*1,2, Natalia Voronina*1, Gustav Steinhoff1,2, Robert David1,2 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University JoVE 57474 Bioengineering
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy Heiko Lemcke1,2,3, Natalia Voronina1,2,3, Gustav Steinhoff1,2,3, Robert David1,2,3 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery, University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock JoVE 55870 Biology
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells Natalia Voronina1, Heiko Lemcke1, Frank Wiekhorst2, Jens-Peter Kühn3, Markus Frank4, Gustav Steinhoff1, Robert David1 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, University of Rostock, 2Physikalisch-Technische Bundesanstalt, 3Department of Radiology and Neuroradiology, Ernst-Moritz-Arndt-University Greifswald, 4Electron Microscopy Center, University of Rostock JoVE 55567 Medicine
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection Christian Rimmbach1, Julia J. Jung1, Robert David1 1Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock JoVE 52465 Developmental Biology
