Seiya Mizuno Laboratory Animal Resource Center and Trans-Border Medical Research Center University of Tsukuba Biography Publications Institution JoVE Articles Seiya Mizuno has not added a biography. If you are Seiya Mizuno and would like to personalize this page please email our Author Liaison for assistance. Publications Coordination Chemogenetics for Activation of GPCR-type Glutamate Receptors in Brain Tissue Nature Communications. Jun, 2022 | Pubmed ID: 35710788 Birth of Mice from Meiotically Arrested Spermatocytes Following Biparental Meiosis in Halved Oocytes EMBO Reports. May, 2022 | Pubmed ID: 35587095 Distinctive High Expression of Antiretroviral APOBEC3 Protein in Mouse Germinal Center B Cells Viruses. 04, 2022 | Pubmed ID: 35458563 Generation of a Gal4-dependent Gene Recombination and Illuminating Mouse Experimental Animals. Apr, 2022 | Pubmed ID: 35444103 L17ER4: A Cell-permeable Attenuated Cationic Amphiphilic Lytic Peptide Bioorganic & Medicinal Chemistry. 05, 2022 | Pubmed ID: 35395514 Inducible Systemic Deletion in Mice Leads to Transient Body Weight Loss Upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen Storage International Journal of Molecular Sciences. Mar, 2022 | Pubmed ID: 35328622 Erk5 in Bone Marrow Mesenchymal Stem Cells Regulates Bone Homeostasis by Preventing Osteogenesis in Adulthood Stem Cells (Dayton, Ohio). 04, 2022 | Pubmed ID: 35304894 Thrombin Cleavage of Osteopontin Initiates Osteopontin's Tumor-promoting Activity Journal of Thrombosis and Haemostasis : JTH. 05, 2022 | Pubmed ID: 35108449 DAJIN Enables Multiplex Genotyping to Simultaneously Validate Intended and Unintended Target Genome Editing Outcomes PLoS Biology. 01, 2022 | Pubmed ID: 35041655 Differential Involvement of Programmed Cell Death Ligands in Skin Immune Responses The Journal of Investigative Dermatology. 01, 2022 | Pubmed ID: 34310947 Albino Mice with the Point Mutation at the Tyrosinase Locus Show High Cholesterol Diet-induced NASH Susceptibility Scientific Reports. 11, 2021 | Pubmed ID: 34750345 A Multistate Stem Cell Dynamics Maintains Homeostasis in Mouse Spermatogenesis Cell Reports. 10, 2021 | Pubmed ID: 34686326 Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction Cancers. Aug, 2021 | Pubmed ID: 34439392 Efficient Induction of Proximity-dependent Labelling by Biotin Feeding in BMAL1-BioID Knock-in Mice Journal of Biochemistry. Dec, 2021 | Pubmed ID: 33982090 EXOC1 Plays an Integral Role in Spermatogonia Pseudopod Elongation and Spermatocyte Stable Syncytium Formation in Mice ELife. 05, 2021 | Pubmed ID: 33973520 Disruption of Entire Locus Leads to Embryonic Lethality by Diminished Gene Expression and Enhanced P53 Pathway ELife. 05, 2021 | Pubmed ID: 33949947 Response to Correspondence on "Reproducibility of CRISPR-Cas9 Methods for Generation of Conditional Mouse Alleles: a Multi-center Evaluation" Genome Biology. 04, 2021 | Pubmed ID: 33827648 Induction of Mutant Allele in Neurons in Late Infancy Increases Sleep Need The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. 03, 2021 | Pubmed ID: 33558433 FGF-23 from Erythroblasts Promotes Hematopoietic Progenitor Mobilization Blood. 03, 2021 | Pubmed ID: 33512467 Mast4 Knockout Shows the Regulation of Spermatogonial Stem Cell Self-renewal Via the FGF2/ERM Pathway Cell Death and Differentiation. 05, 2021 | Pubmed ID: 33219327 Characterization of a Bicistronic Knock-in Reporter Mouse Model for Investigating the Role of CABLES2 in Vivo Experimental Animals. Feb, 2021 | Pubmed ID: 32779618 Efficient Production of Large Deletion and Gene Fragment Knock-in Mice Mediated by Genome Editing with Cas9-mouse Cdt1 in Mouse Zygotes Methods (San Diego, Calif.). 07, 2021 | Pubmed ID: 32334080 Activation of CD8 T Cells Accelerates Anti-PD-1 Antibody-induced Psoriasis-like Dermatitis Through IL-6 Communications Biology. 10, 2020 | Pubmed ID: 33060784 Sleep Architecture in Mice Is Shaped by the Transcription Factor AP-2β Genetics. 11, 2020 | Pubmed ID: 32878901 Ablation of Ventral Midbrain/Pons GABA Neurons Induces Mania-like Behaviors with Altered Sleep Homeostasis and Dopamine DR-mediated Sleep Reduction IScience. Jun, 2020 | Pubmed ID: 32563157 Optimizing Charge Switching in Membrane Lytic Peptides for Endosomal Release of Biomacromolecules Angewandte Chemie (International Ed. in English). 11, 2020 | Pubmed ID: 32557993 Reverse Genetics Reveals Single Gene of Every Candidate on Hybrid Sterility, X Chromosome QTL 2 (Hstx2) Are Dispensable for Spermatogenesis Scientific Reports. 06, 2020 | Pubmed ID: 32493902 Loss of the Conserved PKA Sites of SIK1 and SIK2 Increases Sleep Need Scientific Reports. 05, 2020 | Pubmed ID: 32457359 Ribosome Binding Protein GCN1 regulates the Cell Cycle and Cell Proliferation and is Essential for the Embryonic Development of Mice PLoS Genetics. 04, 2020 | Pubmed ID: 32324833 Generation of B6-Ddx4 , a Novel CreERT2 Knock-in Line, for Germ Cell Lineage by CRISPR/Cas9 Genesis (New York, N.Y. : 2000). 07, 2020 | Pubmed ID: 32293787 C-MAF Deletion in Adult C57BL/6J Mice Induces Cataract Formation and Abnormal Differentiation of Lens Fiber Cells Experimental Animals. Apr, 2020 | Pubmed ID: 31969519 Highly Efficient CRISPR-targeting of the Murine Hipp11 Intergenic Region Supports Inducible Human Transgene Expression Molecular Biology Reports. Feb, 2020 | Pubmed ID: 31811500 Uncovering the Role of MAFB in Glucagon Production and Secretion in Pancreatic α-cells Using a New α-cell-specific Mafb Conditional Knockout Mouse Model Experimental Animals. Apr, 2020 | Pubmed ID: 31787710 Regulation of Bile Acid Metabolism in Mouse Models with Hydrophobic Bile Acid Composition Journal of Lipid Research. 01, 2020 | Pubmed ID: 31645370 RNF208, an Estrogen-inducible E3 Ligase, Targets Soluble Vimentin to Suppress Metastasis in Triple-negative Breast Cancers Nature Communications. 12, 2019 | Pubmed ID: 31862882 Clec10a Regulates Mite-induced Dermatitis Science Immunology. 12, 2019 | Pubmed ID: 31811054 Non-invasive in Vivo Imaging of UCP1 Expression in Live Mice Via Near-infrared Fluorescent Protein IRFP720 PloS One. 2019 | Pubmed ID: 31730675 Reproducibility of CRISPR-Cas9 Methods for Generation of Conditional Mouse Alleles: a Multi-center Evaluation Genome Biology. 08, 2019 | Pubmed ID: 31446895 Lysophosphatidic Acid-induced YAP/TAZ Activation Promotes Developmental Angiogenesis by Repressing Notch Ligand Dll4 The Journal of Clinical Investigation. 07, 2019 | Pubmed ID: 31335323 Generation of Bicistronic Reporter Knockin Mice for Visualizing Germ Layers Experimental Animals. Nov, 2019 | Pubmed ID: 31189761 The Chromatin-binding Protein Phf6 Restricts the Self-renewal of Hematopoietic Stem Cells Blood. 06, 2019 | Pubmed ID: 30917958 KOnezumi: a Web Application for Automating Gene Disruption Strategies to Generate Knockout Mice Bioinformatics (Oxford, England). 09, 2019 | Pubmed ID: 30726877 Generating Vegfr3 Reporter Transgenic Mouse Expressing Membrane-tagged Venus for Visualization of VEGFR3 Expression in Vascular and Lymphatic Endothelial Cells PloS One. 2019 | Pubmed ID: 30601868 Competition for Mitogens Regulates Spermatogenic Stem Cell Homeostasis in an Open Niche Cell Stem Cell. 01, 2019 | Pubmed ID: 30581080 Mice Harboring an MCTO Mutation Exhibit Renal Failure Resembling Nephropathy in Human Patients Experimental Animals. Feb, 2019 | Pubmed ID: 30369533 A Single Phosphorylation Site of SIK3 Regulates Daily Sleep Amounts and Sleep Need in Mice Proceedings of the National Academy of Sciences of the United States of America. 10, 2018 | Pubmed ID: 30254177 De Novo Mutations Activating Germline TP53 in an Inherited Bone-Marrow-Failure Syndrome American Journal of Human Genetics. 09, 2018 | Pubmed ID: 30146126 ZMYND10 Functions in a Chaperone Relay During Axonemal Dynein Assembly ELife. 06, 2018 | Pubmed ID: 29916806 A Mutation in Transcription Factor MAFB Causes Focal Segmental Glomerulosclerosis with Duane Retraction Syndrome Kidney International. 08, 2018 | Pubmed ID: 29779709 Enhanced Antibody Responses in a Novel NOG Transgenic Mouse with Restored Lymph Node Organogenesis Frontiers in Immunology. 2017 | Pubmed ID: 29387068 Simple Generation of Hairless Mice for in Vivo Imaging Experimental Animals. Oct, 2017 | Pubmed ID: 28717054 SHISA6 Confers Resistance to Differentiation-Promoting Wnt/β-Catenin Signaling in Mouse Spermatogenic Stem Cells Stem Cell Reports. 03, 2017 | Pubmed ID: 28196692 Forward-genetics Analysis of Sleep in Randomly Mutagenized Mice Nature. 11, 2016 | Pubmed ID: 27806374 Aberrant PD-L1 Expression Through 3'-UTR Disruption in Multiple Cancers Nature. 06, 2016 | Pubmed ID: 27281199 Germline Recombination in a Novel Cre Transgenic Line, Prl3b1-Cre Mouse Genesis (New York, N.Y. : 2000). 07, 2016 | Pubmed ID: 27124574 Generation of CRISPR/Cas9-mediated Bicistronic Knock-in Ins1-cre Driver Mice Experimental Animals. Jul, 2016 | Pubmed ID: 27053096 Ground-based Assessment of JAXA Mouse Habitat Cage Unit by Mouse Phenotypic Studies Experimental Animals. May, 2016 | Pubmed ID: 26822934 Peri-implantation Lethality in Mice Carrying Megabase-scale Deletion on 5qc3.3 is Caused by Exoc1 Null Mutation Scientific Reports. Sep, 2015 | Pubmed ID: 26346620 Simple Generation of Albino C57BL/6J Mice with G291T Mutation in the Tyrosinase Gene by the CRISPR/Cas9 System Mammalian Genome : Official Journal of the International Mammalian Genome Society. Aug, 2014 | Pubmed ID: 24879364 Generation and Characterization of Ins1-cre-driver C57BL/6N for Exclusive Pancreatic Beta Cell-specific Cre-loxP Recombination Experimental Animals. 2014 | Pubmed ID: 24770644 Truncated Cables1 Causes Agenesis of the Corpus Callosum in Mice Laboratory Investigation; a Journal of Technical Methods and Pathology. Mar, 2014 | Pubmed ID: 24336072 Novel ROSA26 Cre-reporter Knock-in C57BL/6N Mice Exhibiting Green Emission Before and Red Emission After Cre-mediated Recombination Experimental Animals. 2013 | Pubmed ID: 24172193 Retrotransposon-mediated Fgf5(go-Utr) Mutant Mice with Long Pelage Hair Experimental Animals. 2011 | Pubmed ID: 21512271 Effect of Different Culture Conditions on Establishment of Embryonic Stem Cells from BALB/cAJ and NZB/BINJ Mice Cellular Reprogramming. Dec, 2010 | Pubmed ID: 20977302 A Novel Locus on Proximal Chromosome 18 Associated with Agenesis of the Corpus Callosum in Mice Mammalian Genome : Official Journal of the International Mammalian Genome Society. Dec, 2010 | Pubmed ID: 20967545 Embryonic Stem Cells Derived from C57BL/6J and C57BL/6N Mice Comparative Medicine. Aug, 2008 | Pubmed ID: 18724776 Starvation-induced Transcription Factor CREBH Negatively Governs Body Growth by Controlling GH Signaling FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. | Pubmed ID: 34042217 Hyperlipidemia and Hepatitis in Liver-specific CREB3L3 Knockout Mice Generated Using a One-step CRISPR/Cas9 System Scientific Reports. | Pubmed ID: 27291420 Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice Yoko Tanimoto*1, Natsuki Mikami*1,2, Miyuki Ishida1, Natsumi Iki1, Kanako Kato1, Fumihiro Sugiyama1, Satoru Takahashi1, Seiya Mizuno1 1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba JoVE 64161 Developmental Biology
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice Yoko Tanimoto*1, Natsuki Mikami*1,2, Miyuki Ishida1, Natsumi Iki1, Kanako Kato1, Fumihiro Sugiyama1, Satoru Takahashi1, Seiya Mizuno1 1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba JoVE 64161 Developmental Biology