Articles by Simon H. Long in JoVE

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

JoVE 2166 12/07/2010

Alex H. Tuttle*, Matthew M. Rankin*, Monica Teta, Daniel J. Sartori, Geneva M. Stein, Gina J. Kim, Cristina Virgilio, Anne Granger, Di Zhou, Simon H. Long, Alisa B. Schiffman, Jake A. Kushner

Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics, University of Pennsylvania-School of Medicine

We have derived a strategy to detect sequential incorporation of thymidine analogues (CldU and IdU) into tissues of adult mice to quantify two successive rounds of cell division. This strategy is useful to detect cell turnover of long-lived tissues, oncogenic transformation, or transit-amplifying cells.