Stephen C. De Rosa Vaccine and Infectious Disease Division Fred Hutchinson Cancer Research Center Biography Publications Institution JoVE Articles Stephen C. De Rosa has not added a biography. If you are Stephen C. De Rosa and would like to personalize this page please email our Author Liaison for assistance. Publications HIV-1-specific ELISpot Responses in HIV-1 Exposed, Uninfected Partners in Discordant Relationships, Compared to Low-Risk Controls Clinical and Vaccine Immunology : CVI. Sep, 2012 | Pubmed ID: 22971780 Silibinin Inhibits HIV-1 Infection by Reducing Cellular Activation and Proliferation PloS One. 2012 | Pubmed ID: 22848626 Optimization and Qualification of a Multiplex Bead Array to Assess Cytokine and Chemokine Production by Vaccine-specific Cells Journal of Immunological Methods. Aug, 2012 | Pubmed ID: 22626638 A Brilliant New Addition to the Fluorescent Probe Toolbox Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Jun, 2012 | Pubmed ID: 22488984 Immune-correlates Analysis of an HIV-1 Vaccine Efficacy Trial The New England Journal of Medicine. Apr, 2012 | Pubmed ID: 22475592 DNA and Modified Vaccinia Virus Ankara Vaccines Encoding Multiple Cytotoxic and Helper T-lymphocyte Epitopes of Human Immunodeficiency Virus Type 1 (HIV-1) Are Safe but Weakly Immunogenic in HIV-1-uninfected, Vaccinia Virus-naive Adults Clinical and Vaccine Immunology : CVI. May, 2012 | Pubmed ID: 22398243 Vaccine Applications of Flow Cytometry Methods (San Diego, Calif.). Jul, 2012 | Pubmed ID: 22251671 Human Adenovirus-specific T Cells Modulate HIV-specific T Cell Responses to an Ad5-vectored HIV-1 Vaccine The Journal of Clinical Investigation. Jan, 2012 | Pubmed ID: 22201684 A Phase IIA Randomized Clinical Trial of a Multiclade HIV-1 DNA Prime Followed by a Multiclade RAd5 HIV-1 Vaccine Boost in Healthy Adults (HVTN204) PloS One. 2011 | Pubmed ID: 21857901 HIV-DNA Priming Alters T Cell Responses to HIV-adenovirus Vaccine Even when Responses to DNA Are Undetectable Journal of Immunology (Baltimore, Md. : 1950). Sep, 2011 | Pubmed ID: 21844392 A Trimeric, V2-deleted HIV-1 Envelope Glycoprotein Vaccine Elicits Potent Neutralizing Antibodies but Limited Breadth of Neutralization in Human Volunteers The Journal of Infectious Diseases. Apr, 2011 | Pubmed ID: 21451004 Association Between Peripheral γδ T-cell Profile and Disease Progression in Individuals Infected with HIV-1 or HIV-2 in West Africa Journal of Acquired Immune Deficiency Syndromes (1999). Jun, 2011 | Pubmed ID: 21423026 A Phase I Trial of Preventive HIV Vaccination with Heterologous Poxviral-vectors Containing Matching HIV-1 Inserts in Healthy HIV-uninfected Subjects Vaccine. Feb, 2011 | Pubmed ID: 21216311 Safety and Immunogenicity of a Replication-defective Adenovirus Type 5 HIV Vaccine in Ad5-seronegative Persons: a Randomized Clinical Trial (HVTN 054) PloS One. 2010 | Pubmed ID: 21048953 Diversity in CD8(+) T Cell Function and Epitope Breadth Among Persons with Genital Herpes Journal of Clinical Immunology. Sep, 2010 | Pubmed ID: 20635156 ORFeome Approach to the Clonal, HLA Allele-specific CD4 T-cell Response to a Complex Pathogen in Humans Journal of Immunological Methods. Aug, 2009 | Pubmed ID: 19520082 The Cytolytic Enzymes Granyzme A, Granzyme B, and Perforin: Expression Patterns, Cell Distribution, and Their Relationship to Cell Maturity and Bright CD57 Expression Journal of Leukocyte Biology. Jan, 2009 | Pubmed ID: 18820174 HIV-1 Vaccine-induced Immunity in the Test-of-concept Step Study: a Case-cohort Analysis Lancet. Nov, 2008 | Pubmed ID: 19012957 Development of an Automated Analysis System for Data from Flow Cytometric Intracellular Cytokine Staining Assays from Clinical Vaccine Trials Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Sep, 2008 | Pubmed ID: 18615598 Nine-color Flow Cytometry for Accurate Measurement of T Cell Subsets and Cytokine Responses. Part I: Panel Design by an Empiric Approach Cytometry. Part A : the Journal of the International Society for Analytical Cytology. May, 2008 | Pubmed ID: 18383316 Nine-color Flow Cytometry for Accurate Measurement of T Cell Subsets and Cytokine Responses. Part II: Panel Performance Across Different Instrument Platforms Cytometry. Part A : the Journal of the International Society for Analytical Cytology. May, 2008 | Pubmed ID: 18383309 Gamma Delta+ T Cells Involvement in Viral Immune Control of Chronic Human Herpesvirus 8 Infection Journal of Immunology (Baltimore, Md. : 1950). Mar, 2008 | Pubmed ID: 18292568 Optimization and Validation of an 8-color Intracellular Cytokine Staining (ICS) Assay to Quantify Antigen-specific T Cells Induced by Vaccination Journal of Immunological Methods. May, 2007 | Pubmed ID: 17451739 Does Preserving Memory Correlate with Surviving HIV? Trends in Microbiology. Jan, 2007 | Pubmed ID: 17112728 Preservation of T Cell Proliferation Restricted by Protective HLA Alleles is Critical for Immune Control of HIV-1 Infection Journal of Immunology (Baltimore, Md. : 1950). Nov, 2006 | Pubmed ID: 17082660 Quantum Dot Semiconductor Nanocrystals for Immunophenotyping by Polychromatic Flow Cytometry Nature Medicine. Aug, 2006 | Pubmed ID: 16862156 HIV Nonprogressors Preferentially Maintain Highly Functional HIV-specific CD8+ T Cells Blood. Jun, 2006 | Pubmed ID: 16467198 Characterization of Subsets of CD4+ Memory T Cells Reveals Early Branched Pathways of T Cell Differentiation in Humans Proceedings of the National Academy of Sciences of the United States of America. May, 2005 | Pubmed ID: 15905333 Multicolor Immunophenotyping: Human Mature Immune System Methods in Cell Biology. 2004 | Pubmed ID: 15603443 Vaccination in Humans Generates Broad T Cell Cytokine Responses Journal of Immunology (Baltimore, Md. : 1950). Nov, 2004 | Pubmed ID: 15494483 Flow Cytometric Analysis of Vaccine Responses: How Many Colors Are Enough? Clinical Immunology (Orlando, Fla.). Mar, 2004 | Pubmed ID: 15047198 A Potent Anti-HIV Immunotoxin Blocks Spreading Infection by Primary HIV Type 1 Isolates in Multiple Cell Types AIDS Research and Human Retroviruses. Feb, 2004 | Pubmed ID: 15018701 Ontogeny of Gamma Delta T Cells in Humans Journal of Immunology (Baltimore, Md. : 1950). Feb, 2004 | Pubmed ID: 14734745 Sensitive and Viable Identification of Antigen-specific CD8+ T Cells by a Flow Cytometric Assay for Degranulation Journal of Immunological Methods. Oct, 2003 | Pubmed ID: 14580882 Optimized Lymphocyte Isolation Methods for Analysis of Chemokine Receptor Expression Journal of Immunological Methods. Aug, 2003 | Pubmed ID: 12969561 Beyond Six Colors: a New Era in Flow Cytometry Nature Medicine. Jan, 2003 | Pubmed ID: 12514723 Human Immunodeficiency Virus Type 1 Neutralization Measured by Flow Cytometric Quantitation of Single-round Infection of Primary Human T Cells Journal of Virology. May, 2002 | Pubmed ID: 11967298 Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay Tiffany R. Hensley1, Austin B. Easter1, Sarah E. Gerdts1, Stephen C. De Rosa1, Antje Heit1, M. Juliana McElrath1, Erica Andersen-Nissen1 1Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center JoVE 4302 Immunology and Infection
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay Tiffany R. Hensley1, Austin B. Easter1, Sarah E. Gerdts1, Stephen C. De Rosa1, Antje Heit1, M. Juliana McElrath1, Erica Andersen-Nissen1 1Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center JoVE 4302 Immunology and Infection