Stephen P. Muench

Publications

• Tyrosine 288 in the Extracellular Domain of the Human P2X7 Receptor is Critical for Receptor Function Revealed by Structural Modeling and Site-directed Mutagenesis
  Proteins. Oct, 2021  |   Pubmed ID: 34622987
• On-grid and In-flow Mixing for Time-resolved Cryo-EM
  Acta Crystallographica. Section D, Structural Biology. Oct, 2021  |   Pubmed ID: 34605427
• Targeting K1.1 Channels in KCNT1-associated Epilepsy
  Trends in Pharmacological Sciences. 08, 2021  |   Pubmed ID: 34074526
• Structure of the Endocytic Adaptor Complex Reveals the Basis for Efficient Membrane Anchoring During Clathrin-mediated Endocytosis
  Nature Communications. 05, 2021  |   Pubmed ID: 34001871
• Cryo-EM Structure of Human Mitochondrial HSPD1
  IScience. Jan, 2021  |   Pubmed ID: 33506187
• Structural Basis for Vascular Endothelial Growth Factor Receptor Activation and Implications for Disease Therapy
  Biomolecules. 12, 2020  |   Pubmed ID: 33333800
• Human TRPC5 Structures Reveal Interaction of a Xanthine-based TRPC1/4/5 Inhibitor with a Conserved Lipid Binding Site
  Communications Biology. 11, 2020  |   Pubmed ID: 33230284
• Contribution of Val/Ile87 Residue in the Extracellular Domain in Agonist-induced Current Responses of the Human and Rat P2X7 Receptors
  Purinergic Signalling. 12, 2020  |   Pubmed ID: 33029714
• Need for Speed: Examining Protein Behavior During CryoEM Grid Preparation at Different Timescales
  Structure (London, England : 1993). 11, 2020  |   Pubmed ID: 32814033
• Potent Tetrahydroquinolone Eliminates Apicomplexan Parasites
  Frontiers in Cellular and Infection Microbiology. 2020  |   Pubmed ID: 32626661
• Cryo-EM Structure and Molecular Dynamics Analysis of the Fluoroquinolone Resistant Mutant of the AcrB Transporter from
  Microorganisms. Jun, 2020  |   Pubmed ID: 32585951
• The Active Form of Quinol-dependent Nitric Oxide Reductase from is a Dimer
  IUCrJ. May, 2020  |   Pubmed ID: 32431824
• Structure-Based Identification and Characterization of Inhibitors of the Epilepsy-Associated K1.1 (KCNT1) Potassium Channel
  IScience. May, 2020  |   Pubmed ID: 32408169
• Structure of the Protective Nematode Protease Complex H-gal-GP and Its Conservation Across Roundworm Parasites
  PLoS Pathogens. 04, 2020  |   Pubmed ID: 32271834
• Sample Deposition Onto Cryo-EM Grids: from Sprays to Jets and Back
  Acta Crystallographica. Section D, Structural Biology. Apr, 2020  |   Pubmed ID: 32254058
• Styrene Maleic-acid Lipid Particles (SMALPs) into Detergent or Amphipols: An Exchange Protocol for Membrane Protein Characterisation
  Biochimica Et Biophysica Acta. Biomembranes. 05, 2020  |   Pubmed ID: 31945320
• Unprecedented Properties of Phenothiazines Unraveled by a NDH-2 Bioelectrochemical Assay Platform
  Journal of the American Chemical Society. 01, 2020  |   Pubmed ID: 31880924
• Styrene Maleic Acid Recovers Proteins from Mammalian Cells and Tissues While Avoiding Significant Cell Death
  Scientific Reports. 11, 2019  |   Pubmed ID: 31767876
• A Cryo-EM Grid Preparation Device for Time-resolved Structural Studies
  IUCrJ. Nov, 2019  |   Pubmed ID: 31709058
• Structural Insight into Eukaryotic Sterol Transport Through Niemann-Pick Type C Proteins
  Cell. 10, 2019  |   Pubmed ID: 31543266
• Dimeric Structures of Quinol-dependent Nitric Oxide Reductases (qNORs) Revealed by Cryo-electron Microscopy
  Science Advances. 08, 2019  |   Pubmed ID: 31489376
• Emerging Role of Electron Microscopy in Drug Discovery
  Trends in Biochemical Sciences. 10, 2019  |   Pubmed ID: 31409563
• LAT1 (SLC7A5) and CD98hc (SLC3A2) Complex Dynamics Revealed by Single-particle Cryo-EM
  Acta Crystallographica. Section D, Structural Biology. Jul, 2019  |   Pubmed ID: 31282475
• SMA-PAGE: A New Method to Examine Complexes of Membrane Proteins Using SMALP Nano-encapsulation and Native Gel Electrophoresis
  Biochimica Et Biophysica Acta. Biomembranes. 08, 2019  |   Pubmed ID: 31150633
• The Expanding Toolkit for Structural Biology: Synchrotrons, X-ray Lasers and CryoEM
  IUCrJ. Mar, 2019  |   Pubmed ID: 30867914
• Substrate Polyspecificity and Conformational Relevance in ABC Transporters: New Insights from Structural Studies
  Biochemical Society Transactions. 12, 2018  |   Pubmed ID: 30514765
• CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in
  Frontiers in Cellular and Infection Microbiology. 2018  |   Pubmed ID: 30345257
• Cryo-EM Structures of Complex I from Mouse Heart Mitochondria in Two Biochemically Defined States
  Nature Structural & Molecular Biology. 07, 2018  |   Pubmed ID: 29915388
• Approaches to Altering Particle Distributions in Cryo-electron Microscopy Sample Preparation
  Acta Crystallographica. Section D, Structural Biology. 06, 2018  |   Pubmed ID: 29872006
• X-ray and Cryo-EM Structures of Inhibitor-bound Cytochrome Complexes for Structure-based Drug Discovery
  IUCrJ. Mar, 2018  |   Pubmed ID: 29765610
• No Longer Hidden Secrets of Proton Pumping: The Resolution Revolution Enlightens V-ATPases
  Molecular Cell. 03, 2018  |   Pubmed ID: 29547720
• Elucidating the Structural Basis for Differing Enzyme Inhibitor Potency by Cryo-EM
  Proceedings of the National Academy of Sciences of the United States of America. 02, 2018  |   Pubmed ID: 29434040
• A Reconstitution Method for Integral Membrane Proteins in Hybrid Lipid-polymer Vesicles for Enhanced Functional Durability
  Methods (San Diego, Calif.). 09, 2018  |   Pubmed ID: 29410153
• Using a SMALP Platform to Determine a Sub-nm Single Particle Cryo-EM Membrane Protein Structure
  Biochimica Et Biophysica Acta. Feb, 2018  |   Pubmed ID: 28993151
• AtSPX1 Affects the AtPHR1-DNA-binding Equilibrium by Binding Monomeric AtPHR1 in Solution
  The Biochemical Journal. 10, 2017  |   Pubmed ID: 28887383
• Everyone Needs Good Neighbours - the Intricate Relationship Between the Acetylcholine-receptor Channel and Its Membrane Environment
  IUCrJ. Jul, 2017  |   Pubmed ID: 28875018
• Durable Vesicles for Reconstitution of Membrane Proteins in Biotechnology
  Biochemical Society Transactions. Feb, 2017  |   Pubmed ID: 28202656
• Conformational Changes During Human P2X7 Receptor Activation Examined by Structural Modelling and Cysteine-based Cross-linking Studies
  Purinergic Signalling. 03, 2017  |   Pubmed ID: 28025718
• The Conservation of Phosphate-binding Residues Among PHT1 Transporters Suggests That Distinct Transport Affinities Are Unlikely to Result from Differences in the Phosphate-binding Site
  Biochemical Society Transactions. 10, 2016  |   Pubmed ID: 27911737
• The Changing Landscape of Membrane Protein Structural Biology Through Developments in Electron Microscopy
  Molecular Membrane Biology. 03, 2016  |   Pubmed ID: 27608730
• Durable Proteo-hybrid Vesicles for the Extended Functional Lifetime of Membrane Proteins in Bionanotechnology
  Chemical Communications (Cambridge, England). Sep, 2016  |   Pubmed ID: 27540604
• Structure-based Identification and Characterisation of Structurally Novel Human P2X7 Receptor Antagonists
  Biochemical Pharmacology. 09, 2016  |   Pubmed ID: 27481062
• Artificial Membranes for Membrane Protein Purification, Functionality and Structure Studies
  Biochemical Society Transactions. 06, 2016  |   Pubmed ID: 27284055
• The Varied Functions of Aluminium-activated Malate Transporters-much More Than Aluminium Resistance
  Biochemical Society Transactions. 06, 2016  |   Pubmed ID: 27284052
• Rotating with the Brakes on and Other Unresolved Features of the Vacuolar ATPase
  Biochemical Society Transactions. 06, 2016  |   Pubmed ID: 27284051
• Pi Sensing and Signalling: from Prokaryotic to Eukaryotic Cells
  Biochemical Society Transactions. 06, 2016  |   Pubmed ID: 27284040
• A Method for Detergent-free Isolation of Membrane Proteins in Their Local Lipid Environment
  Nature Protocols. 07, 2016  |   Pubmed ID: 27254461
• Corrigendum to 'Methods to Account for Movement and Flexibility in Cryo-EM Data Processing' [Methods 100 (2016) 35-41]
  Methods (San Diego, Calif.). 07, 2016  |   Pubmed ID: 27209563
• Characterization of the Flexibility of the Peripheral Stalk of Prokaryotic Rotary A-ATPases by Atomistic Simulations
  Proteins. 09, 2016  |   Pubmed ID: 27177595
• Editorial
  Methods (San Diego, Calif.). 05, 2016  |   Pubmed ID: 27060404
• Methods to Account for Movement and Flexibility in Cryo-EM Data Processing
  Methods (San Diego, Calif.). 05, 2016  |   Pubmed ID: 27016144
• An Introduction to Sample Preparation and Imaging by Cryo-electron Microscopy for Structural Biology
  Methods (San Diego, Calif.). May, 2016  |   Pubmed ID: 26931652
• Extracellular and Luminal PH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes
  The Journal of Biological Chemistry. Apr, 2016  |   Pubmed ID: 26912656
• In Situ Formation of Magnetopolymersomes Via Electroporation for MRI
  Scientific Reports. Sep, 2015  |   Pubmed ID: 26391797
• Docking of Competitive Inhibitors to the P2X7 Receptor Family Reveals Key Differences Responsible for Changes in Response Between Rat and Human
  Bioorganic & Medicinal Chemistry Letters. Aug, 2015  |   Pubmed ID: 26099538
• Mechanism of Inhibition of Mouse Slo3 (KCa 5.1) Potassium Channels by Quinine, Quinidine and Barium
  British Journal of Pharmacology. Sep, 2015  |   Pubmed ID: 26045093
• Replace, Reuse, Recycle: Improving the Sustainable Use of Phosphorus by Plants
  Journal of Experimental Botany. Jun, 2015  |   Pubmed ID: 25944926
• Structure of the Vacuolar H+-ATPase Rotary Motor Reveals New Mechanistic Insights
  Structure (London, England : 1993). Mar, 2015  |   Pubmed ID: 25661654
• The Acanthamoeba Shikimate Pathway Has a Unique Molecular Arrangement and is Essential for Aromatic Amino Acid Biosynthesis
  Protist. Feb, 2015  |   Pubmed ID: 25576842
• The Use of SMALPs As a Novel Membrane Protein Scaffold for Structure Study by Negative Stain Electron Microscopy
  Biochimica Et Biophysica Acta. Feb, 2015  |   Pubmed ID: 25450810
• Understanding the Apparent Stator-rotor Connections in the Rotary ATPase Family Using Coarse-grained Computer Modeling
  Proteins. Dec, 2014  |   Pubmed ID: 25174610
• Non-synonymous Single Nucleotide Polymorphisms in the P2X Receptor Genes: Association with Diseases, Impact on Receptor Functions and Potential Use As Diagnosis Biomarkers
  International Journal of Molecular Sciences. Jul, 2014  |   Pubmed ID: 25079442
• PA1b Inhibitor Binding to Subunits C and E of the Vacuolar ATPase Reveals Its Insecticidal Mechanism
  The Journal of Biological Chemistry. Jun, 2014  |   Pubmed ID: 24795045
• ALOX12 in Human Toxoplasmosis
  Infection and Immunity. Jul, 2014  |   Pubmed ID: 24686056
• The Benzimidazole Based Drugs Show Good Activity Against T. Gondii but Poor Activity Against Its Proposed Enoyl Reductase Enzyme Target
  Bioorganic & Medicinal Chemistry Letters. Feb, 2014  |   Pubmed ID: 24398298
• Subunit Positioning and Stator Filament Stiffness in Regulation and Power Transmission in the V1 Motor of the Manduca Sexta V-ATPase
  Journal of Molecular Biology. Jan, 2014  |   Pubmed ID: 24075871
• Flexibility Within the Rotor and Stators of the Vacuolar H+-ATPase
  PloS One. 2013  |   Pubmed ID: 24312643
• Discrimination of Potent Inhibitors of Toxoplasma Gondii Enoyl-acyl Carrier Protein Reductase by a Thermal Shift Assay
  Biochemistry. Dec, 2013  |   Pubmed ID: 24295325
• Modification of Triclosan Scaffold in Search of Improved Inhibitors for Enoyl-acyl Carrier Protein (ACP) Reductase in Toxoplasma Gondii
  ChemMedChem. Jul, 2013  |   Pubmed ID: 23776166
• Development of a Triclosan Scaffold Which Allows for Adaptations on Both the A- and B-ring for Transport Peptides
  Bioorganic & Medicinal Chemistry Letters. Jun, 2013  |   Pubmed ID: 23664871
• Design, Synthesis, and Biological Activity of Diaryl Ether Inhibitors of Toxoplasma Gondii Enoyl Reductase
  Bioorganic & Medicinal Chemistry Letters. Apr, 2013  |   Pubmed ID: 23453069
• Novel N-benzoyl-2-hydroxybenzamide Disrupts Unique Parasite Secretory Pathway
  Antimicrobial Agents and Chemotherapy. May, 2012  |   Pubmed ID: 22354304
• Structural Divergence of the Rotary ATPases
  Quarterly Reviews of Biophysics. Aug, 2011  |   Pubmed ID: 21426606
• T. Gondii RP Promoters & Knockdown Reveal Molecular Pathways Associated with Proliferation and Cell-cycle Arrest
  PloS One. 2010  |   Pubmed ID: 21124925
• Archazolid A Binds to the Equatorial Region of the C-ring of the Vacuolar H+-ATPase
  The Journal of Biological Chemistry. Dec, 2010  |   Pubmed ID: 20884613
• Identification of T. Gondii Epitopes, Adjuvants, and Host Genetic Factors That Influence Protection of Mice and Humans
  Vaccine. May, 2010  |   Pubmed ID: 20347630
• Cryo-electron Microscopy of the Vacuolar ATPase Motor Reveals Its Mechanical and Regulatory Complexity
  Journal of Molecular Biology. Mar, 2009  |   Pubmed ID: 19244615