Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Epithelial Effects of Intestinal Inflammation on Established Murine Colonoids

June 2nd, 2023
*1, *2,3, 2,3, 2,3
1Division of Pediatric Gastroenterology, Hepatology and Nutrition, UPMC Children’s Hospital of Pittsburgh, 2Division of Pediatric Surgery, UPMC Children’s Hospital of Pittsburgh, 3Department of Surgery, University of Pittsburgh School of Medicine
* These authors contributed equally

Summary
Automatic Translation

Vi beskriver en protokoll som beskriver isolering av murine kolonkrypter for utvikling av 3-dimensjonale kolonoider. De etablerte kolonoidene kan deretter terminalt differensieres for å reflektere den cellulære sammensetningen av vertsepitelet før de mottar en inflammatorisk utfordring eller blir rettet mot å etablere et epitelmonolag.

Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter