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De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

October 20th, 2023
1,2, 1,2, 1,2,3, 4,5, 6, 1,2,7
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology, Albert Einstein College of Medicine, 5Department of Oncology, Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Summary
Automatic Translation

يتم تقديم بروتوكول فريد وشامل لتوليد خلايا متغصنة مشتقة من الخلايا الأحادية البشرية (mo-DCs) من خلايا الدم أحادية النواة المحيطية المعزولة (PBMCs) باستخدام علاج sialidase. علاوة على ذلك ، يتم وصف طرق تقييم التوصيف الظاهري والوظيفي ل mo-DCs وتقييم كيفية تحسين علاج sialidase لمستوى نضج mo-DCs.

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