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De-Sialylated Monocyte-Derived-DCs from Isolated PBMCs Using Sialidase Treatment

October 20th, 2023
1,2, 1,2, 1,2,3, 4,5, 6, 1,2,7
1Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 3LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 4Department of Microbiology and Immunology, Albert Einstein College of Medicine, 5Department of Oncology, Albert Einstein College of Medicine, 6Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark, Zona Industrial da Gandra, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa

Summary
Automatic Translation

Er wordt een uniek, uitgebreid protocol gepresenteerd voor het genereren van gedesialyleerde humane monocyt-afgeleide dendritische cellen (mo-DC's) uit geïsoleerde perifere mononucleaire bloedcellen (PBMC's) met behulp van een sialidasebehandeling. Verder worden methoden beschreven om de fenotypische en functionele karakterisering van mo-DC's te beoordelen en te evalueren hoe behandeling met sialidase het rijpingsniveau van mo-DC's verbetert.

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