A high-throughput method to globally study the organelle morphology in S. cerevisiae

JoVE 1224 3/02/2009

Shabnam Tavassoli, Jesse Tzu-Cheng Chao, Christopher Loewen

Department of Cellular and Physiological Sciences, University of British Columbia - UBC

GFP-fusion proteins are widely used to visualize organelles by confocal microscopy. However, screening for mutations that affect the morphology of organelles generally requires individual mutagenesis and is time consuming. Here, we demonstrate a method to simultaneously incorporate organelle-GFP markers in almost 5,000 non-essential genes in yeast.