JoVE Applied Physics
1MESA+ Institute for Nanotechnology, University of Twente
Procedures are outlined to prepare segmented and coaxial nanowires via templated electrodeposition in nanopores. As examples, segmented nanowires consisting of Ag and ZnO segments, and coaxial nanowires consisting of a TiO2 shell and a Ag core were made. The nanowires were used in photocatalytic hydrogen formation experiments.
Published May 2, 2014. Keywords: Physics, Multicomponent nanowires, electrochemistry, sol-gel processes, photocatalysis, photochemistry, H2 evolution
JoVE Applied Physics
1Department of Physics, University of Ottawa, 2Ottawa-Carleton Institute of Biomedical Engineering, University of Ottawa
A methodology for preparing solid-state nanopores in solution for biomolecular translocation experiments is presented. By applying short pulses of high electric fields, the nanopore diameter can be controllably enlarged with subnanometer precision and its electrical noise characteristics significantly improved. This procedure is performed in situ using standard laboratory equipment under experimental conditions.
Published October 31, 2013. Keywords: Physics, Nanopore, Solid-State, Size Control, Noise Reduction, Translocation, DNA, High Electric Fields, Nanopore Conditioning
1Department of Pediatrics, Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill
Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.
Published September 11, 2014. Keywords: Biophysics, Freeze-fracture; Freeze-etch; Membranes; Intercellular junctions; Materials science; Nanotechnology; Electron microscopy
1Department of Physics, Worcester Polytechnic Institute, 2Department of Chemical Engineering, Worcester Polytechnic Institute
This paper demonstrates a protocol to characterize the mechanical properties of living cells by means of microindentation using an Atomic Force Microscope (AFM).
Published June 27, 2013. Keywords: Biophysics, Bioengineering, Cellular Biology, Molecular Biology, Physics, Chemical Engineering, Biomechanics, bioengineering (general), AFM, cell stiffness, microindentation, force spectroscopy, atomic force microscopy, microscopy
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
We present a combination of Cryo-electron microscopy, lipid nanotechnology, and structure analysis applied to resolve the membrane-bound structure of two highly homologous FVIII forms: human and porcine. The methodology developed in our laboratory to helically organize the two functional recombinant FVIII forms on negatively charged lipid nanotubes (LNT) is described.
Published June 3, 2014. Keywords: Bioengineering, Cryo-electron microscopy, Lipid nanotubes, Helical assembly, Membrane-bound organization, Coagulation factor VIII
JoVE Applied Physics
1Department of Energy and NEMAS - Center for NanoEngineered Materials and Surfaces, Politecnico di Milano, 2Center for Nano Science and Technology, Instituto Italiano di Tecnologia
We describe the experimental method to deposit nanostructured oxide thin films by nanosecond Pulsed Laser Deposition (PLD) in the presence of a background gas. By using this method Al-doped ZnO (AZO) films, from compact to hierarchically structured as nano-tree forests, can be deposited.
Published February 27, 2013. Keywords: Materials Science, Physics, Nanotechnology, Nanoengineering, Oxides, thin films, thin film theory, deposition and growth, Pulsed laser Deposition (PLD), Transparent conducting oxides (TCO), Hierarchically organized Nanostructured oxides, Al doped ZnO (AZO) films, enhanced light scattering capability, gases, deposition, nanoporus, nanoparticles, Van der Pauw, scanning electron microscopy, SEM
1Department of Electrical and Computer Engineering, University of California, Davis, 2Department of Chemical Engineering and Materials Science, University of California, Davis, 3Department of Biomedical Engineering, University of California, Davis
We report on techniques to micropattern nanoporous gold thin films via stencil printing and photolithography, as well as methods to culture cells on the microfabricated patterns. In addition, we describe image analysis methods to characterize morphology of the material and the cultured cells using scanning electron and fluorescence microscopy techniques.
Published July 15, 2013. Keywords: Bioengineering, Cellular Biology, Molecular Biology, Biomedical Engineering, Biochemistry, Chemistry, Chemical Engineering, Biophysics, Physics, Nanotechnology, Nanostructures, Biomedical Technology, Miniaturization, Gold, Staining and Labeling, Cell Culture Techniques, Microscopy, Electron Microscopy, Fluorescence, Nanotechnology, thin films (theory, deposition and growth), Nanoporous gold, cell culture, image analysis, microfabrication, nanotechnology, quantitative immunochemistry, scanning electron microscopy, SEM, fluorescence microscopy, stencil printing, photolithography, cell culture
1Faculty of Life Sciences and the Institute for Nanotechnology & Advanced Materials, Bar-Ilan University
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here, we describe how DNA origami can be utilized to design a robotic robot capable of sensing biological cues and responding by shape shifting, subsequently relayed to a desired effect.
Published July 8, 2013. Keywords: Bioengineering, Genetics, Biomedical Engineering, Molecular Biology, Medicine, Genomics, Nanotechnology, Nanomedicine, DNA origami, nanorobot, caDNAno, DNA, DNA Origami, nucleic acids, DNA structures, CAD, sequencing
JoVE Immunology and Infection
1London Centre for Nanotechnology and Departments of Medicine, University College London
Acquired resistance to antibiotics is a major public healthcare problem and is presently ranked by the WHO as one of the greatest threats to human life. Here we describe the use of cantilever technology to quantify antibacterial resistance, critical to the discovery of novel and powerful agents against multidrug resistant bacteria.
Published October 25, 2013. Keywords: Immunology, Engineering, Technology, Diagnostic Techniques and Procedures, Early Diagnosis, Bacterial Infections and Mycoses, Lipids, Amino Acids, Peptides, and Proteins, Chemical Actions and Uses, Diagnosis, Therapeutics, Surface stress, vancomycin, mucopeptides, cantilever sensor
1Department of Physics and Astronomy, The University of Texas at San Antonio, 2Centro de Investigaciones en Optica A. C., 3Department of Biology and Neurosciences Institute, The University of Texas at San Antonio
We synthesized star shaped gold nanostars using a silver seed mediated growth method. The diameter of the nanostars ranges from 200 to 300 nm and the number of tips vary from 7 to 10. The nanoparticles have a broad surface plasmon resonance mode centered in the near infrared.
Published January 15, 2012. Keywords: Bioengineering, thermal ablation, surface plasmon resonance, nanoparticle, nanotechnology, silver seeds