1Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University
The protocols here describe kinetic assays of protein-protein interactions with Bio-layer Interferometry. F-type ATP synthase, which is involved in cellular energy metabolism, can be inhibited by its ε subunit in bacteria. We have adapted Bio-layer Interferometry to study interactions of the catalytic complex with ε’s inhibitory C-terminal domain.
Published February 18, 2014. Keywords: Chemistry, ATP synthase, Bio-Layer Interferometry, Ligand-induced conformational change, Biomolecular Interaction Analysis, Allosteric regulation, Enzyme inhibition
1Fischell Department of Bioengineering, University of Maryland, 2Institute for Bioscience and Biotechnology Research, University of Maryland, 3Department of Materials Science and Engineering, University of Maryland
This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.
Published June 6, 2012. Keywords: Bioengineering, Biomedical Engineering, electrodeposition, biofabrication, chitosan, alginate, lab-on-a-chip, microfluidic, DTRA
1Dept of Physics, MIT - Massachusetts Institute of Technology, 2Department of Electrical Engineering and Computer Science, MIT - Massachusetts Institute of Technology
We demonstrate a simple method for placing cells at desired locations on a substrate. This method patterns cells by flipping a silicone chip containing microwells filled with cells onto the substrate. This method provides a new way to modulate diffusible and juxtacrine signaling between cells.
Published October 1, 2007. Keywords: Cellular Biology, tissue engineering, stem cells, patterning, bioengineering, signaling, diffusible, autocrine, juxtacrine
1Shimadzu, Scientific Instruments
This communication presents data on the accuracy and reproducibility of the BioSpec-nano UV-VIS spectrophotometer for dsDNA and protein quantitation. Even with ultra-small volumes (1 to 2 L), reproducibility is excellent, while the automated wiping function improves throughput and results in minimal carryover for more precise results.
Published February 17, 2011. Keywords: Molecular Biology, Nucleic acid quantitation, protein quantitation, micro-volume analysis, label quantitation
1Department of Developmental and Cell Biology, University of California, Irvine (UCI), 2Department of Pathology, University of California, Irvine (UCI)
Published October 31, 2007. Keywords: Basic Protocols, RNA, Purification, Brain
JoVE Application Notes
Published July 12, 2009. Keywords: Advertisement, Experion, Experion automated electrophoresis system, video product demonstration, product demo, RNA run, analysis data, electropherogram, software, total RNA, mRNA, assays, RQI, quality indicator, virtual gels
JoVE Application Notes
Published June 15, 2011. Keywords: Bio-Plex, Multi-Plex, Cytokines, Amino Assays, Bio-Rad, XMAP, Luminex
1Faculty of Life Sciences and the Institute for Nanotechnology & Advanced Materials, Bar-Ilan University
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here, we describe how DNA origami can be utilized to design a robotic robot capable of sensing biological cues and responding by shape shifting, subsequently relayed to a desired effect.
Published July 8, 2013. Keywords: Bioengineering, Genetics, Biomedical Engineering, Molecular Biology, Medicine, Genomics, Nanotechnology, Nanomedicine, DNA origami, nanorobot, caDNAno, DNA, DNA Origami, nucleic acids, DNA structures, CAD, sequencing
1Department of Biomedical Engineering, McGill University, Montreal, QC Canada
Nanoparticles such as semiconductor quantum dots (QDs) can be used to create photoactivatable agents for anti-microbial or anti-cancer applications. This technique shows how to water-solubilize cadmium telluride (CdTe) QDs, conjugate them to an antibiotic, and perform a bacterial inhibition assay based upon growth curves and plate count.
Published July 11, 2012. Keywords: Biomedical Engineering, Bioengineering, Molecular Biology, Quantum dots, solubilization, conjugation, cytotoxicity, phototoxicity, growth curve, plate count
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University
An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.
Published September 21, 2011. Keywords: Biochemistry, hydrophobic interaction chromatography, liquid chromatography, green fluorescent protein, GFP, scouting, protein purification, Bio-Rad DuoFlow, FPLC