Solid Phase Synthesis of a Functionalized Bis-Peptide Using …
Published 5/15/2012
Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
1Department of Pediatric Oncology, University Children's Hospital Essen, 2Department of Developmental Pathology, Bonn Medical School, Institute of Pathology
To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.
Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
1Institute for Physiological Chemistry, Department of Biochemical Endocrinology, University of Duisburg-Essen, 2Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen, 3Morphoplant GmbH, 4ARCONS Institute for Applied Research and Didactics
The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.
Method for Culture of Early Chick Embryos ex vivo (New Culture)
1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
In Ovo Electroporation in Embryonic Chick Retina
1Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Department of Biomedical Engineering, Rutgers University
The overall goal of this video is to show how to perform targeted retinal injection and in ovo electroporation of DNA/RNA constructs into the chick embryonic retina at the Hamburger and Hamilton stage 22-23, which is about embryonic day 4 (E4). This technique is very useful to study gene expression, gene regulation, and morphological change in developing chick retina.
Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
Department of Biochemistry and Cell Biology, Rice University
An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
1Department of Biomedical Engineering, Cornell University, 2Current Address: Mechanical Engineering Department, Dogus University
In this article, we present a simple methodology to enable long-term ex-ovo avian embryo culture. This technique is ideal for longitudinal experimentation requiring complete optical accessibility and/or sterile transportation in avian embryos.
Method for Whole Mount Antibody Staining in Chick
Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)
This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.
Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development
Department of Orthopaedic Surgery, University of California San Francisco
This article describes a tissue transplantation technique that was designed to test the signaling and patterning properties of surface cephalic ectoderm during craniofacial development.
Assay for Neural Induction in the Chick Embryo
Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)
Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.
Xenotransplantation of Human Stem Cells into the Chicken Embryo
1Department of Physiology, Oregon Health and Science University, 2Norwegian Center for Stem Cell Research, Oregon Health and Science University
In this paper we present a method for transplanting human stem cells into various regions of the central nervous system of the chicken embryo. This provides an in vivo model for assessing the proliferation and differentiation of various types of human stem cells in embryonic tissue environments.
More Results...