Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

JoVE 1286 7/27/2009

Shahin Zangenehpour^1,2, Mark W. Burke², Avi Chaudhuri³, Maurice Ptito²

¹Cognitive Neuroscience Unit, Montreal Neurological Institute, ²Ècole d’Optomètrie, Universitè de Montrèal, ³Department of Psychology, McGill University

Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.