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JoVE Neuroscience
JoVE Neuroscience is a multidisciplinary section devoted to investigations of the structure, function, physiology, and pathophysiology of the brain and nervous system. Included methodologies range from molecular and cellular level studies to full central and peripheral neural systems. Potential treatment platforms and surgical techniques for neurological diseases and disorders are also presented in this section.
 JoVE Neuroscience

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

1VA Palo Alto Health Care System, 2Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 3Department of Physical Therapy, Arkansas State University


JoVE 52371

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.

 JoVE Neuroscience

Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array

1Neural Engineering Center, Department of Biomedical Engineering, Case Western Reserve University


JoVE 52601

We have developed an in vitro unfolded hippocampus which preserves CA1-CA3 array of neurons. Combined with the penetrating micro-electrode array, neural activity can be monitored in both the longitudinal and transverse orientations. This method provides advantages over hippocampal slice preparations as the propagation in the entire hippocampus can be recorded simultaneously.

 JoVE Neuroscience

Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

1Department of Clinical Neurosciences, Hotchkiss Brain Institute at University of Calgary, 2Department of Oncology, Hotchkiss Brain Institute at University of Calgary


JoVE 52679

Demyelinating diseases can be modeled in animals by focal application of lysolecithin into the CNS. A single injection of lysolecithin into mouse spinal cord produces a lesion that spontaneously repairs over time. The goal is to study factors involved in de- and remyelination, and to test agents for enhancing repair.

 JoVE Neuroscience

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

1MSTP, Neuroscience Program, Washington University in St. Louis, 2Washington University Pain Center, Department of Anesthesiology, Washington University in St. Louis


JoVE 52640

The Cold Plantar Assay (CPA) measures cold responsiveness between 30 °C and 5 °C, and can also measure cold adaptation. This protocol describes how to use the CPA to measure cold hypersensitivity, analgesia, and adaptation in mice.

 JoVE Neuroscience

Electroretinogram Analysis of the Visual Response in Zebrafish Larvae

1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, 2Department of Ophthalmology, Duke University


JoVE 52662

We present a method for the electroretinographic (ERG) analysis of zebrafish larvae utilizing micromanipulation and electroretinography techniques. This is a simple and straightforward method for assaying visual function of zebrafish larvae in vivo.

 JoVE Neuroscience

Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

1Department of Otolaryngology-Head and Neck Surgery, University of California, San Francisco


JoVE 52187

The described post-auricular surgical approach allows rapid and direct delivery into the mouse cochlear scala tympani while minimizing blood loss and animal mortality. This method can be used for cochlear therapy using molecular, pharmacologic and viral delivery to postnatal mice through the round window membrane.

 JoVE Neuroscience

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

1Department of Neurosurgery, Brigham & Women's Hospital, Harvard Medical School, 2Division of Brain Sciences, Department of Medicine, Imperial College London


JoVE 52457

The mechanism underlying the therapeutic effects of Deep Brain Stimulation (DBS) surgery needs investigation. The methods presented in this manuscript describe an experimental approach to examine the cellular events triggered by DBS by analyzing the gene expression profile of candidate genes that can facilitate neurogenesis post DBS surgery.

 JoVE Neuroscience

Closed-loop Neuro-robotic Experiments to Test Computational Properties of Neuronal Networks

1Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia


JoVE 52341

In this paper, an experimental framework to perform closed-loop experiments is presented, in which information processing (i.e., coding and decoding) and learning of neuronal assemblies are studied during the continuous interaction with a robotic body.

 JoVE Neuroscience

Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging

1Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Turin, 2Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, 3Bracco Research Center, Bracco Imaging SpA


JoVE 52162

The present work describes a new protocol to perform non-invasive high-frequency ultrasound and photoacoustic based imaging on rat brain, to efficiently visualize deep subcortical regions and their vascular patterns by directing signals on skull foramina naturally present on animal cranium.

 JoVE Neuroscience

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

1Centre for Neuroprosthesis, EPFL | STI | IMT/IBI | LSBI, 2Blond McIndoe Research Laboratories, Institute of Inflammation & Repair, The University of Manchester, 3University Hospital of South Manchester


JoVE 52543

Dorsal root ganglia (DRG) are structures containing the sensory neurons of the peripheral nervous system. When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site.

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