JoVE Neuroscience is a multidisciplinary section devoted to investigations of the structure, function, physiology, and pathophysiology of the brain and nervous system. Included methodologies range from molecular and cellular level studies to full central and peripheral neural systems. Potential treatment platforms and surgical techniques for neurological diseases and disorders are also presented in this section.
1Department of Otology and Laryngology, Harvard Medical School, 2Department of Communication Sciences and Disorders, Worcester State University
The goal of this protocol is to outline a surgical approach to provide direct access to the dorsal cochlear nucleus in a murine model.
Published January 20, 2015. Keywords: Neuroscience, Optogenetics, dorsal cochlear nucleus, virus-mediated gene transfer, auditory system
1Florey Institute of Neuroscience and Mental Health, The University of Melbourne
This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult mice.
Published January 20, 2015. Keywords: Neuroscience, Behavior, midbrain, tyrosine hydroxylase, dopamine, plasticity, substantia nigra pars compacta
1Department of Molecular Biology and Biochemistry, Simon Fraser University, 2Department of Biomedical Physiology and Kinesiology, Simon Fraser University
This protocol demonstrates how Proximity Ligation Assay can be used to detect in situ protein-protein interactions at the Drosophila larval neuromuscular junction. With this technique, Discs large and Hu-li tai shao are shown to form a complex at the postsynaptic region, an association previously identified through co-immunoprecipitation.
Published January 20, 2015. Keywords: Neuroscience, adducin, body wall dissection, developmental biology, Discs large, Drosophila, Hu-li tai shao, immunohistochemistry, neuromuscular junction, neuroscience, protein-protein interaction, Proximity Ligation Assay, third instar larvae
1Department of Psychiatry, University of Pittsburgh Medical Center, 2Department of Bioengineering, Stanford University, 3Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, 4Department of Neurobiology and Behavior, Cornell University, 5Department of Psychiatry and Behavioral Sciences, Stanford University
Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.
Published January 15, 2015. Keywords: Neuroscience, optogenetics, rodent, behavior, opsin, channelrhodopsin, brain, fiber optics, laser, neural circuits
1Institute of Neuroscience and Medicine (INM-2), Research Centre Jülich, 2Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, JARA, RWTH Aachen University
Patch-clamp recordings and simultaneous intracellular biocytin filling of synaptically coupled neurons in acute brain slices allow a correlated analysis of their structural and functional properties. The aim of this protocol is to describe the essential technical steps of electrophysiological recording from neuronal microcircuits and their subsequent morphological analysis.
Published January 10, 2015. Keywords: Neuroscience, Patch-clamp, paired recordings, neurons, synaptic connections, gap junctions, biocytin labeling, structure-function correlations
1Pharmacology and Toxicology, Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin
Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.
Published December 27, 2014. Keywords: Neuroscience, Olfactory system, cilia, immunohistochemistry, signal transduction, Kirrel, olfactory receptor, mOR-EG, en face preparation
1Physiology and Bosch Institute, University of Sydney, 2Motor Neuron Disease Research Group, Australian School of Advanced Medicine, Macquarie University, 3Advanced Microscopy Facility, Bosch Institute, University of Sydney
The neuromuscular junction (NMJ) is altered in a variety of conditions that can sometimes culminate in synaptic failure. This report describes fluorescence microscope-based methods to quantify such structural changes.
Published December 26, 2014. Keywords: Neuroscience, neuromuscular, motor endplate, motor control, sarcopenia, myasthenia gravis, amyotrophic lateral sclerosis, morphometry, confocal, immunofluorescence
1Institute of Neuro and Behavioral Biology, Westfälische Wilhelms-Universität Münster, 2Department of Mathematics and Computer Science, Westfälische Wilhelms-Universität Münster
FIM is a novel, cost effective imaging system designed to track small moving objects such as C. elegans, planaria or Drosophila larvae. The accompanying FIMTrack program is designed to deliver fast and efficient data analysis. Together, these tools allow high-throughput analysis of behavioral traits.
Published December 24, 2014. Keywords: Neuroscience, Drosophila, C. elegans, locomotion analysis, tracking software, animal behavior, high-throughput, Frustrated Total Internal Reflection (FTIR), FTIR-based Imaging Method (FIM), Neuroscience
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision
Neural retina of a mouse aged 8 days is on top of a 4% gelatin block. After isolation of the photoreceptor layer (200 µm) by vibratome, the photoreceptors are seeded after mechanical and enzymatic dissociation for culture. The photoreceptor layer can be used for molecular, biochemical analyses or transplantation.
Published December 22, 2014. Keywords: Neuroscience, Primary photoreceptor cell culture, Inherited retinal degeneration, Rod-derived Cone Viability Factor, S-Antigen, Flat-mounted mouse retina, Transplantation.
1Department of Pharmacology and Toxicology, University of Saarland School of Medicine
Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.
Published December 22, 2014. Keywords: Neuroscience, developmental biology, neural circuits, transsynaptic tracing, barley lectin, Tetanus Toxin fragment C, mouse embryo, gene targeting, ROSA26 locus, kisspeptin, GnRH, reproduction