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Medicine

En vivo doble sustrato bioluminiscentes imagen

Published: October 11, 2011 doi: 10.3791/3245

Materials

Name Company Catalog Number Comments
pGL4.2-Puro [luc2/Puro] Vector Promega Corp. E6751
Glomax Mult-idection system Promega Corp.
IVIS 200 series Caliper Life Sciences
Dual luciferase reporter Assay system Promega Corp. E1960
Rediject c–lenterazine-h Caliper Life Sciences 760506
D-Luciferin K-Salt Caliper Life Sciences 122796

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References

  1. Wetterwald, A. Optical imaging of cancer metastasis to bone marrow: a mouse model of minimal residual disease. Am. J. Pathol. 160, 1143-1153 (2002).
  2. Wendt, M. K., Drury, L. J., Vongsa, R. A., Dwinell, M. B. Constitutive CXCL12 Expression Induces Anoikis in Colorectal Carcinoma Cells. Gastroenterology. 135, 508-508 (2008).
  3. Wendt, M., Schiemann, W. Therapeutic targeting of the focal adhesion complex prevents oncogenic TGF-beta signaling and metastasis. Breast Cancer Research. 11, R68-R68 (2009).
  4. Korpal, M. Imaging transforming growth factor-beta signaling dynamics and therapeutic response in breast cancer bone metastasis. Nat Med. , (2009).
  5. Giampieri, S. Localized and reversible TGFbeta signalling switches breast cancer cells from cohesive to single cell motility. Nat Cell Biol. 11, 1287-1296 (2009).
  6. Giampieri, S., Pinner, S., Sahai, E. Intravital imaging illuminates transforming growth factor beta signaling switches during metastasis. Cancer Res. 70, 3435-3439 (2010).
  7. DeNardo, D. G. CD4(+) T cells regulate pulmonary metastasis of mammary carcinomas by enhancing protumor properties of macrophages. Cancer Cell. 16, 91-102 (2009).
  8. Wyckoff, J. A Paracrine Loop between Tumor Cells and Macrophages Is Required for Tumor Cell Migration in Mammary Tumors. Cancer Res. 64, 7022-7029 (2004).
  9. Kim, M. Y. Tumor self-seeding by circulating cancer cells. Cell. 139, 1315-1326 (2009).
  10. Aslakson, C. J., Miller, F. R. Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Cancer Res. 52, 1399-1405 (1992).
  11. Wendt, M. K., Smith, J. A., Schiemann, W. P. Transforming growth factor-beta-induced epithelial-mesenchymal transition facilitates epidermal growth factor-dependent breast cancer progression. Oncogene. 29, 6485-6498 (2010).
  12. Hawkins, E., Unch, J., Murhpy, N., Vidugiriene, J., Scurria, M., Klaubert, D., Wood, K. Bright Light, No Lysis. Measuring Renilla Luciferase Luminescence in Living Cells. , Promega. (2005).
  13. Roncali, E. New device for real-time bioluminescence imaging in moving rodents. J. Biomed. Opt. 13, 054035-054035 (2008).
<em>En vivo</em> doble sustrato bioluminiscentes imagen
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Cite this Article

Wendt, M. K., Molter, J., Flask, C.More

Wendt, M. K., Molter, J., Flask, C. A., Schiemann, W. P. In vivo Dual Substrate Bioluminescent Imaging. J. Vis. Exp. (56), e3245, doi:10.3791/3245 (2011).

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