Materials
| Name | Company | Catalog Number | Comments |
| Petri dish, polystyrene, sterile Geriner Bio-one 35 x 10 mm | VWR international | 82050-540 | |
| BD Falcon Cell Strainers, Sterile, BD Biosciences, blue, 40 μm | VWR international | 21008-949 | |
| Hanks’ Balanced Salt Solution (HBSS). Solution with Calcium, Magnesium, and without Phenol Red, Sterile | VWR international | 45000-456 | |
| Cellstar centrifuge tubes, polypropylene, sterile, greiner bio-one, 50 ml | VWR international | 82050-346 | |
| Cellstar centrifuge tubes, polypropylene, sterile, greiner bio-one, 15 ml | VWR international | 82050-278 | |
| B-27 Serum-Free Supplement (50x), liquid | Invitrogen | 17504-044 | |
| N-2 Supplement (100x), liquid | Invitrogen | 17502-048 | |
| bFGF, fibroblast growth factor-basic human | Sigma-Aldrich | F0291 | |
| IGF-1, insulin-like growth factor-1 from mouse | Sigma-Aldrich | I8779 | |
| EGF, epidermal growth factor human | Sigma-Aldrich | E9644 | |
| Dulbecco’s Modified Eagle’s Medium/Ham’s F-12 50/50 Mix: 1X, with L-Glutamine and 15 mM HEPES | VWR international | 45000-350 | |
| BD Falcon Round-Bottom Tubes, Disposable, Polystyrene, 12x75 | VWR international | 60819-295 | |
| Trypsin, 0.25% (1X) with EDTA 4Na, liquid | Invitrogen | 25200-056 | |
| ep Dualfilter TIPS 300uL, 960 TIPS | Eppendorf | 22491245 | |
| Trypsin Inhibitor, Soybean, Purified | Worthington Biochemical | LS003570 | |
| BD Falcon 40um Cell Strainers | BD Biosciences | 21008-949 | |
| Multiwell Plates, Polystyrene, Greiner Bio-One, Nontreated Plates, 6 wells | VWR international | 82050-846 | |
| Marker Gene FACS Blue LacZ beta-Galactosidase Detection Kit | Marker Gene Technologies | M0255 |
References
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- Savary, E. Cochlear stem/progenitor cells from a postnatal cochlea respond to Jagged1 and demonstrate that notch signaling promotes sphere formation and sensory potential. Mech. Dev. 125, 674-686 (2008).
- White, P. M., Doetzlhofer, A., Lee, Y. S., Groves, A. K., Segil, N. Mammalian cochlear supporting cells can divide and trans-differentiate into hair cells. Nature. 441, 984-987 (2006).
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