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Murine Bioluminescent Hepatic Tumour Model

Article DOI: 10.3791/1977-v • 05:24 min • July 17th, 2010
July 17th, 2010 •
1, 1, 2, 2, 3, 1, 1
1Cork Cancer Research Centre, Mercy University Hospital and Leslie C. Quick Jnr. Laboratory, University College Cork, 2Department of Computer Science, University College Cork, 3South Infirmary Victoria University Hospital

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This article describes a procedure for the induction of orthotopic bioluminescent liver tumours in mice, and subsequent analysis of tumour growth confined to the liver using live whole body luminescence imaging.

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Murine Bioluminescent Hepatic Tumour Model Liver Metastases Mouse Model Bioluminescent Imaging Tumour Cells Liver Tumour Spleen Mobilisation Vascular Pedicle Lewis Lung Carcinoma Cells Firefly Luciferase Gene Hemi-spleen Resection Abdomen Bioluminescence Imaging IVIS Whole Body Imaging System Quantitative Imaging Viable Tumour Cells Tumour Cell Death Necrosis Drug Treatment Metastatic Tumour-cell Survival Growth Mechanisms Evaluation Of Therapeutics
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