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JoVE Journal
Biology

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高活性酵母核糖体的色谱分离纯化
 
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高活性酵母核糖体的色谱分离纯化

Article DOI: 10.3791/3214-v 09:14 min October 24th, 2011
October 24th, 2011

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

准备用传统的方法,纯化共同净化核酸酶和蛋白酶的真核细胞的核糖体的污染造成了消极影响下游的生化和结构分析。一个快速和简单的色谱的净化方法是用来解决这个问题作为一个模型系统中使用的酵母核糖体。

Tags

细胞生物学,56期,核糖体,纯化的DNA,酵母,色谱法,
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