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Journal / Bioengineering / Alongamento curtas seqüências de DNA com Pinça…
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JoVE Journal Bioengineering
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Alongamento curtas seqüências de DNA com Pinça força constante óptico axial

Article DOI: 10.3791/3405-v • 08:48 min • October 13th, 2011
October 13th, 2011 •
1, 2, 2
1LSA Biophysics, University of Michigan, 2LSA Biophysics, Department of Physics, University of Michigan

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Ilustramos o uso de uma constante de força axial pinças ópticas para explorar as propriedades mecânicas das moléculas de ADN curtas. Esticando DNA axialmente, minimizamos impedimento estérico e artefatos provenientes de manipulação laterais convencional, o que nos permite estudar moléculas de DNA tão curto quanto ~ 100 nm.

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Emissão de bioengenharia 56 Genética DNA alongamento DNA axial pinças ópticas uma única molécula de Biofísica Biofísica
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