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Velge og isolere kolonier av menneskeskapte pluripotent stamceller omprogrammert fra voksne Fibroblaster
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Velge og isolere kolonier av menneskeskapte pluripotent stamceller omprogrammert fra voksne Fibroblaster

Article DOI: 10.3791/3416-v • 13:24 min • February 20th, 2012
February 20th, 2012 •
1,2, 1, 3, 3, 1, 1
1Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, 2Department of Cell Biology, Poznan University of Medical Sciences, 3Department of Molecular Biology, The Scripps Research Institute

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Vi presenterer en protokoll for effektiv omprogrammering av humane somatiske celler inn i menneskeskapt påvirkning pluripotent stamceller (hiPSC) bruker retrovirale vektorer som koder Oct3 / 4, Sox2, Klf4 og c-myc (OSKM) og identifisering av korrekt omprogrammeres hiPSC av levende farging med Tra- 1-81 antistoff.

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Developmental Biology stamceller induserte pluripotent stamceller iPSC somatisk celle omprogrammering pluripotency retrovirale transduksjon
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