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Journal / Biology / En hurtigere, høj opløsning, MTPA-GFP-baserede…
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Biology

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En hurtigere, høj opløsning, MTPA-GFP-baserede Mitokondriel Fusion Assay Erhvervelse Kinetiske data i flere celler i parallel anvendelse af konfokal mikroskopi
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
Journal (Biology)
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Journal (Biology)
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
 
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En hurtigere, høj opløsning, MTPA-GFP-baserede Mitokondriel Fusion Assay Erhvervelse Kinetiske data i flere celler i parallel anvendelse af konfokal mikroskopi

Article DOI: 10.3791/3991-v • 10:45 min • July 20th, 2012
July 20th, 2012 •
1, 2, 3, 3, 3
1Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, 2Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, 3Department of Medicine, Boston University Medical Center

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Mitokondrie fusion blev målt ved at spore ækvilibrering af photoconverted matrix-målrettet GFP over mitokondriske netværket over tid. Hidtil kunne kun en celle udsættes for en time lange kinetisk analyse ad gangen. Vi præsenterer en metode, der samtidigt måler flere celler, og dermed fremskynde indsamlingen af ​​data.

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Molekylær Biologi Genetik Cellular biologi fysik konfokal mikroskopi mitokondrier fusion TMRE mtPAGFP INS1 mitokondrier dynamik mitokondrier morfologi mitokondriel netværk
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