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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro Ensaio de cocultura para avaliar migração trans epitelial de neutrófilo induzida por patógenos
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In vitro Ensaio de cocultura para avaliar migração trans epitelial de neutrófilo induzida por patógenos

Article DOI: 10.3791/50823-v • 14:14 min • January 6th, 2014
January 6th, 2014 •
1,2,3, 1,3, 3, 1,3
1Department of Pediatrics, Harvard Medical School, 2Department of Pediatric Gastroenterology, MGH for Children, 3Mucosal Immunology and Biology Research Center, Massachusetts General Hospital

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A migração trans-epitelial de neutrófilos em resposta à infecção bacteriana mucosa contribui para lesão epitelial e doença clínica. Foi desenvolvido um modelo in vitro que combina patógenos, neutrófilos humanos e camadas de células epiteliais humanas polarizadas cultivadas em filtros transwell para facilitar investigações para desvendar os mecanismos moleculares que orquestram esse fenômeno.

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Infecção Questão 83 Biologia Celular Epitélio Neutrófilos Pseudomonas aeruginosa Doenças do Trato Respiratório Neutrófilos barreiras epiteliais patógenos transmigração
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