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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro Coculture Assay at vurdere Patogen induceret Neutrophil Trans-epitel Migration
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In vitro Coculture Assay at vurdere Patogen induceret Neutrophil Trans-epitel Migration

Article DOI: 10.3791/50823-v • 14:14 min • January 6th, 2014
January 6th, 2014 •
1,2,3, 1,3, 3, 1,3
1Department of Pediatrics, Harvard Medical School, 2Department of Pediatric Gastroenterology, MGH for Children, 3Mucosal Immunology and Biology Research Center, Massachusetts General Hospital

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Neutrofil trans-epitelmigration som reaktion på slimhindebakteriel infektion bidrager til epitelskader og kliniske sygdomme. En in vitro-model er blevet udviklet, der kombinerer patogen, humane neutrofiler og polariserede menneskelige epitelcellelag dyrket på transwellfiltre for at lette undersøgelser mod optrævling af de molekylære mekanismer, der orkestrerer dette fænomen.

Tags

Infektion Problem 83 Cellulær biologi Epitel Neutrophils Pseudomonas aeruginosa Respiratory Tract Diseases Neutrophils epitelbarrierer patogener transmigration
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