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Journal / Chemistry / Kvantitativa Proteomics Använda Reduktiv…
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JoVE Journal Chemistry
Kvantitativa Proteomics Använda Reduktiv dimetylering för Stable Isotope Labeling
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Kvantitativa Proteomics Använda Reduktiv dimetylering för Stable Isotope Labeling

Article DOI: 10.3791/51416-v • 11:53 min • July 1st, 2014
July 1st, 2014 •
1,2,3, 4
1CEA, DSV, IG, Genoscope, 2CNRS-UMR8030, Évry, France, 3Université d'Évry Val d'Essonne, 4Massachusetts General Hospital Cancer Center

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Stabil isotopmärkning av peptider genom reduktiv dimetylering (redi märkning) är en snabb, billig strategi för noggranna masspektrometri-baserad kvantitativa proteomik. Här visar vi en robust metod för beredning och analys av proteinblandningar med hjälp av redi strategi som kan tillämpas på nästan alla prov typ.

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Kemi kvantitativa proteomik masspektrometri stabil isotop reduktiv dimetylering peptidmärkning LC-MS/MS
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