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Journal / Developmental Biology / Ex vivo Culture of Chick lillehjernen Slices og…
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Developmental Biology

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JoVE Journal Developmental Biology
Ex vivo Culture of Chick lillehjernen Slices og Romlig Målrettet Electroporation av granulat Cell Forstadier
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Ex vivo Culture of Chick lillehjernen Slices og Romlig Målrettet Electroporation av granulat Cell Forstadier

Article DOI: 10.3791/53421-v • 10:02 min • December 14th, 2015
December 14th, 2015 •
1, 1, 2
1MRC Centre of Developmental Neurobiology, King's College London, 2School of Biological and Chemical Sciences, Queen Mary, University of London

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Lillehjernen eksterne granulat laget er åsted for det største transitt forsterkning i utviklingen av hjernen. Her presenterer vi en protokoll for å målrette genmodifisering til dette laget på toppen av spredning ved hjelp av ex vivo elektroporering og kulturen i lillehjernen skiver fra embryonale Day 14 kyllingfoster.

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Developmental Biology lillehjernen utvikling chick ekstern granulat lag skive kultur granule celler Purkinje celler elektroporering.
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