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Journal / Developmental Biology / Ex Vivo Kultur Chick cerebellare Skiver og…
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Developmental Biology

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JoVE Journal Developmental Biology
Ex Vivo Kultur Chick cerebellare Skiver og Rumligt Målrettet Elektroporation af granulat celleforstadier
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Ex Vivo Kultur Chick cerebellare Skiver og Rumligt Målrettet Elektroporation af granulat celleforstadier

Article DOI: 10.3791/53421-v • 10:02 min • December 14th, 2015
December 14th, 2015 •
1, 1, 2
1MRC Centre of Developmental Neurobiology, King's College London, 2School of Biological and Chemical Sciences, Queen Mary, University of London

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Den cerebellar eksterne granulat lag er stedet for den største transit forstærkning i den udviklende hjerne. Her præsenterer vi en protokol til at målrette genetisk modifikation til dette lag på toppen af spredning ved hjælp af ex vivo elektroporation og kultur i cerebellare skiver fra embryonale Dag 14 kyllingeembryoer.

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Developmental Biology lillehjernen udvikling chick ekstern granulat lag skive kultur granula celler Purkinjeceller elektroporation.
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